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  Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser

Kupitz, C., Basu, S., Grotjohann, I., Fromme, R., Zatsepin, N. A., Rendek, K. N., et al. (2014). Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser. Nature, 513(7517), 261-265. doi:10.1038/nature13453.

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Genre: Journal Article

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Kupitz, Christopher, Author
Basu, Shibom, Author
Grotjohann, Ingo, Author
Fromme, Raimund, Author
Zatsepin, Nadia A., Author
Rendek, Kimberly N., Author
Hunter, Mark S., Author
Shoeman, Robert L.1, Author              
White, Thomas A., Author
Wang, Dingjie, Author
James, Daniel, Author
Yang, Jay-How, Author
Cobb, Danielle E., Author
Reeder, Brenda, Author
Sierra, Raymond G., Author
Liu, Haiguang, Author
Barty, Anton, Author
Aquila, Andrew L., Author
Deponte, Daniel, Author
Kirian, Richard A., Author
Bari, Sadia, AuthorBergkamp, Jesse J., AuthorBeyerlein, Kenneth R., AuthorBogan, Michael J., AuthorCaleman, Carl, AuthorChao, Tzu-Chiao, AuthorConrad, Chelsie E., AuthorDavis, Katherine M., AuthorFleckenstein, Holger, AuthorGalli, Lorenzo, AuthorHau-Riege, Stefan P., AuthorKassemeyer, Stephan1, Author              Laksmono, Hartawan, AuthorLiang, Mengning, AuthorLomb, Lukas1, Author              Marchesini, Stefano, AuthorMartin, Andrew V., AuthorMesserschmidt, Marc, AuthorMilathianaki, Despina, AuthorNass, Karol1, Author              Ros, Alexandra, AuthorRoy-Chowdhury, Shatabdi, AuthorSchmidt, Kevin, AuthorSeibert, Marvin, AuthorSteinbrener, Jan1, Author              Stellato, Francesco, AuthorYan, Lifen, AuthorYoon, Chunhong, AuthorMoore, Thomas A., AuthorMoore, Ana L., AuthorPushkar, Yulia, AuthorWilliams, Garth J., AuthorBoutet, Sébastien, AuthorDoak, R. Bruce, AuthorWeierstall, Uwe, AuthorFrank, Matthias, AuthorChapman, Henry N., AuthorSpence, John C. H., AuthorFromme, Petra, Author more..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Photosynthesis, a process catalysed by plants, algae and cyanobacteria converts sunlight to energy thus sustaining all higher life on Earth. Two large membrane protein complexes, photosystem I and II (PSI and PSII), act in series to catalyse the light-driven reactions in photosynthesis. PSII catalyses the light-driven water splitting process, which maintains the Earth’s oxygenic atmosphere1. In this process, the oxygen-evolving complex (OEC) of PSII cycles through five states, S0 to S4, in which four electrons are sequentially extracted from the OEC in four light-driven charge-separation events. Here we describe time resolved experiments on PSII nano/microcrystals from Thermosynechococcus elongatus performed with the recently developed2 technique of serial femtosecond crystallography. Structures have been determined from PSII in the dark S1 state and after double laser excitation (putative S3 state) at 5 and 5.5 Å resolution, respectively. The results provide evidence that PSII undergoes significant conformational changes at the electron acceptor side and at the Mn4CaO5 core of the OEC. These include an elongation of the metal cluster, accompanied by changes in the protein environment, which could allow for binding of the second substrate water molecule between the more distant protruding Mn (referred to as the ‘dangler’ Mn) and the Mn3CaOx cubane in the S2 to S3 transition, as predicted by spectroscopic and computational studies3, 4. This work shows the great potential for time-resolved serial femtosecond crystallography for investigation of catalytic processes in biomolecules.

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Language(s): eng - English
 Dates: 2013-11-262014-05-042014-07-092014-09-11
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nature13453
Other: 7994
 Degree: -

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Title: Nature
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 513 (7517) Sequence Number: - Start / End Page: 261 - 265 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238