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  alpha-SNAP interferes with the zippering of the SNARE protein membrane fusion machinery.

Park, Y., Vennekate, W., Yavuz, H., Preobraschenski, J., Hernandez, J. M., Riedel, D., et al. (2014). alpha-SNAP interferes with the zippering of the SNARE protein membrane fusion machinery. Journal of Biological Chemistry, 289(23), 16326-16335. doi:10.1074/jbc.M114.556803.

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Datensatz-Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-F94D-2 Versions-Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-3D71-5
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 Urheber:
Park, Y.1, Autor              
Vennekate, W.2, Autor              
Yavuz, H.1, Autor              
Preobraschenski, J.1, Autor              
Hernandez, J. M.1, Autor              
Riedel, D.3, Autor              
Walla, P. J.2, Autor              
Jahn, R.1, Autor              
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              
2Research Group of Biomolecular Spectroscopy and Single-Molecule Detection, MPI for biophysical chemistry, Max Planck Society, ou_578565              
3Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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 Zusammenfassung: Neuronal exocytosis is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. Before fusion, SNARE proteins form complexes bridging the membrane followed by assembly toward the C-terminal membrane anchors, thus initiating membrane fusion. After fusion, the SNARE complex is disassembled by the AAA-ATPase N-ethylmaleimide-sensitive factor that requires the cofactor alpha-SNAP to first bind to the assembled SNARE complex. Using chromaffin granules and liposomes we now show that alpha-SNAP on its own interferes with the zippering of membrane-anchored SNARE complexes midway through the zippering reaction, arresting SNAREs in a partially assembled trans-complex and preventing fusion. Intriguingly, the interference does not result in an inhibitory effect on synaptic vesicles, suggesting that membrane properties also influence the final outcome of alpha-SNAP interference with SNARE zippering. We suggest that binding of alpha-SNAP to the SNARE complex affects the ability of the SNARE complex to harness energy or transmit force to the membrane.

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Sprache(n): eng - Englisch
 Datum: 2014-04-282014-05-05
 Publikationsstatus: Im Druck veröffentlicht
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1074/jbc.M114.556803
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Titel: Journal of Biological Chemistry
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 289 (23) Artikelnummer: - Start- / Endseite: 16326 - 16335 Identifikator: -