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  Fluorogenic probes for live-cell imaging of the cytoskeleton.

Lukinavicius, G., Reymond, L., D'Este, E., Masharina, A., Göttfert, F., Ta, H., et al. (2014). Fluorogenic probes for live-cell imaging of the cytoskeleton. Nature Methods, 11(7), 731-733. doi:10.1038/nmeth.2972.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-001A-0415-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-4839-1
Genre: Journal Article

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 Creators:
Lukinavicius, G., Author
Reymond, L., Author
D'Este, E.1, Author              
Masharina, A., Author
Göttfert, F.1, Author              
Ta, H.1, Author              
Guether, A., Author
Fournier, M., Author
Rizzo, S., Author
Waldmann, H., Author
Blaukopf, C., Author
Sommer, C., Author
Gerlich, D. W., Author
Arndt, H. D., Author
Hell, S. W.1, Author              
Johnsson, K., Author
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.

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Language(s): eng - English
 Dates: 2014-05-252014-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nmeth.2972
 Degree: -

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Title: Nature Methods
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 11 (7) Sequence Number: - Start / End Page: 731 - 733 Identifier: -