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  STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.

Ilgen, P., Stoldt, S., Conradi, L. C., Wurm, C. A., Rüschoff, J., Ghadimi, B. M., et al. (2014). STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue. PLoS One, 9(7): e101563. doi:10.1371/journal.pone.0101563.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-001A-0823-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CC8C-E
Genre: Journal Article

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 Creators:
Ilgen, P., Author
Stoldt, S.1, Author              
Conradi, L. C., Author
Wurm, C. A.2, Author              
Rüschoff, J., Author
Ghadimi, B. M., Author
Liersch, T., Author
Jakobs, S.1, Author              
Affiliations:
1Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              
2Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Formalin fixed and paraffin-embedded human tissue resected during cancer surgery is indispensable for diagnostic and therapeutic purposes and represents a vast and largely unexploited resource for research. Optical microscopy of such specimen is curtailed by the diffraction-limited resolution of conventional optical microscopy. To overcome this limitation, we used STED super-resolution microscopy enabling optical resolution well below the diffraction barrier. We visualized nanoscale protein distributions in sections of well-annotated paraffin-embedded human rectal cancer tissue stored in a clinical repository. Using antisera against several mitochondrial proteins, STED microscopy revealed distinct sub-mitochondrial protein distributions, suggesting a high level of structural preservation. Analysis of human tissues stored for up to 17 years demonstrated that these samples were still amenable for super-resolution microscopy. STED microscopy of sections of HER2 positive rectal adenocarcinoma revealed details in the surface and intracellular HER2 distribution that were blurred in the corresponding conventional images, demonstrating the potential of super-resolution microscopy to explore the thus far largely untapped nanoscale regime in tissues stored in biorepositories.

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Language(s): eng - English
 Dates: 2014-07-15
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0101563
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Title: PLoS One
Source Genre: Journal
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Pages: 8 Volume / Issue: 9 (7) Sequence Number: e101563 Start / End Page: - Identifier: -