English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Miniaturization of two-photon microscopy for imaging in freely moving animals

Helmchen, F., Denk, W., & Kerr, J. (2013). Miniaturization of two-photon microscopy for imaging in freely moving animals. Cold Spring Harbor Protocols, 2013(10), 904-913. doi:10.1101/pdb.top078147.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-001A-12F6-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-3DBB-9
Genre: Journal Article

Files

show Files

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Helmchen, F, Author
Denk, W, Author
Kerr, JND1, 2, Author              
Affiliations:
1Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497807              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              

Content

show
hide
Free keywords: -
 Abstract: This article describes the development and application of miniaturized two-photon-excited fluorescence microscopes ("two-photon fiberscopes"). Two-photon fiberscopes have been developed with the aim of enabling high-resolution imaging of neural activity in freely behaving animals. They use fiber optics to deliver laser light for two-photon excitation. Their small front piece typically contains a miniature scanning mechanism and imaging optics. Two-photon fiberscopes can be made sufficiently small and lightweight to be carried by rats and mice and to allow virtually unrestricted movement within a behavioral arena. Typically mounted to the animal's skull above a cranial window, two-photon fiberscopes permit imaging of cells down to at least 250 m below the brain surface (e.g., in rat neocortex). In freely exploring animals, action-potential-evoked calcium transients can be imaged in individual somata of visual cortex neurons bulk-labeled with a calcium indicator. Two-photon fiberscopes thus enable high-resolution optical recording of neural activity with cellular resolution during natural behaviors.

Details

show
hide
Language(s):
 Dates: 2013-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1101/pdb.top078147
BibTex Citekey: HelmchenDK2013
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cold Spring Harbor Protocols
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 2013 (10) Sequence Number: - Start / End Page: 904 - 913 Identifier: -