English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803

Kabisch, A., Otto, A., König, S., Becher, D., Albrecht, D., Schüler, M., et al. (2014). Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME JOURNAL, 8(7), 1492-1502. doi:10.1038/ismej.2014.4.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-001A-1A10-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-001A-1A12-6
Genre: Journal Article

Files

show Files
hide Files
:
ismej20144a.pdf (Any fulltext), 2MB
Name:
ismej20144a.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
open access article
License:
-

Locators

show

Creators

show
hide
 Creators:
Kabisch, Antje1, Author
Otto, Andreas1, Author
König, Sten1, Author
Becher, Dörte1, Author
Albrecht, Dirk1, Author
Schüler, Margarete2, Author              
Teeling, Hanno1, Author
Amann, Rudolf I.1, Author
Schweder, Thomas1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

Content

show
hide
Free keywords: OUTER-MEMBRANE PROTEINS; QUANTITATIVE-ANALYSIS; BACILLUS-SUBTILIS; GENOME ANALYSIS; GUT MICROBIOTA; SOFTWARE TOOL; CELL-SURFACE; ALGINIC ACID; BROWN-ALGAE; BACTERIAalginate; Bacteroidetes; laminarin; polysaccharide utilization locus; TonB-dependent transporter; proteomics;
 Abstract: Members of the phylum Bacteroidetes are abundant in many marine ecosystems and are known to have a pivotal role in the mineralization of complex organic substrates such as polysaccharides and proteins. We studied the decomposition of the algal glycans laminarin and alginate by 'Gramella forsetii' KT0803, a bacteroidetal isolate from North Sea surface waters. A combined application of isotope labeling, subcellular protein fractionation and quantitative proteomics revealed two large polysaccharide utilization loci (PULs) that were specifically induced, one by alginate and the other by laminarin. These regulons comprised genes of surface-exposed proteins such as oligomer transporters, substrate-binding proteins, carbohydrate-active enzymes and hypothetical proteins. Besides, several glycan-specific TonB-dependent receptors and SusD-like substrate-binding proteins were expressed also in the absence of polysaccharide substrates, suggesting an anticipatory sensing function. Genes for the utilization of the beta-1,3-glucan laminarin were found to be co-regulated with genes for glucose and alpha-1,4-glucan utilization, which was not the case for the non-glucan alginate. Strong syntenies of the PULs of 'G. forsetii' with similar loci in other Bacteroidetes indicate that the specific response mechanisms of 'G. forsetii' to changes in polysaccharide availability likely apply to other Bacteroidetes. Our results can thus contribute to an improved understanding of the ecological niches of marine Bacteroidetes and their roles in the polysaccharide decomposition part of carbon cycling in marine ecosystems.

Details

show
hide
Language(s): eng - English
 Dates: 2014-07
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: ISI: 000338213900013
DOI: 10.1038/ismej.2014.4
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ISME JOURNAL
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 8 (7) Sequence Number: - Start / End Page: 1492 - 1502 Identifier: ISSN: 1751-7362