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  Souffle/Spastizin controls secretory vesicle maturation during zebrafish oogenesis.

Kanagaraj, P., Gautier-Stein, A., Riedel, D., Schomburg, C., Cerda, J., Vollack, N., et al. (2014). Souffle/Spastizin controls secretory vesicle maturation during zebrafish oogenesis. PLoS Genetics, 10(6): e1004449. doi:10.1371/journal.pgen.1004449.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-001A-20C2-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CC92-D
Genre: Journal Article

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Kanagaraj, P., Author
Gautier-Stein, A., Author
Riedel, D.1, Author              
Schomburg, C., Author
Cerda, J., Author
Vollack, N., Author
Dosch, R., Author
Affiliations:
1Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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 Abstract: During oogenesis, the egg prepares for fertilization and early embryogenesis. As a consequence, vesicle transport is very active during vitellogenesis, and oocytes are an outstanding system to study regulators of membrane trafficking. Here, we combine zebrafish genetics and the oocyte model to identify the molecular lesion underlying the zebrafish souffle (suf) mutation. We demonstrate that suf encodes the homolog of the Hereditary Spastic Paraplegia (HSP) gene SPASTIZIN (SPG15). We show that in zebrafish oocytes suf mutants accumulate Rab11b-positive vesicles, but trafficking of recycling endosomes is not affected. Instead, we detect Suf/Spastizin on cortical granules, which undergo regulated secretion. We demonstrate genetically that Suf is essential for granule maturation into secretion competent dense-core vesicles describing a novel role for Suf in vesicle maturation. Interestingly, in suf mutants immature, secretory precursors accumulate, because they fail to pinch-off Clathrin-coated buds. Moreover, pharmacological inhibition of the abscission regulator Dynamin leads to an accumulation of immature secretory granules and mimics the suf phenotype. Our results identify a novel regulator of secretory vesicle formation in the zebrafish oocyte. In addition, we describe an uncharacterized cellular mechanism for Suf/Spastizin activity during secretion, which raises the possibility of novel therapeutic avenues for HSP research.

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Language(s): eng - English
 Dates: 2014-06-26
 Publication Status: Published online
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1371/journal.pgen.1004449
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Title: PLoS Genetics
Source Genre: Journal
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Pages: 17 Volume / Issue: 10 (6) Sequence Number: e1004449 Start / End Page: - Identifier: -