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  Scaffolding the expansion of autophagosomes

Kaufmann, A., & Wollert, T. (2014). Scaffolding the expansion of autophagosomes. AUTOPHAGY, 10(7), 1343-1345. doi:10.4161/auto.28980.

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 Urheber:
Kaufmann, Anna1, Autor           
Wollert, Thomas1, Autor           
Affiliations:
1Wollert, Thomas / Molecular Membrane and Organelle Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565175              

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Schlagwörter: macroautophagy; Atg8; conjugation; reconstitution; membrane scaffold;
 Zusammenfassung: The conjugation of the small ubiquitin (Ub)-like protein Atg8 to autophagic membranes is a key step during the expansion of phagophores. This reaction is driven by 2 interconnected Ub-like conjugation systems. The second system conjugates the Ub-like protein Atg12 to Atg5. The resulting conjugate catalyzes the covalent attachment of Atg8 to membranes. Atg12-Atg5, however, constitutively associates with the functionally less well-characterized coiled-coil protein Atg16. By reconstituting the conjugation of Atg8 to membranes in vitro, we showed that after Atg8 has been attached to phosphatidylethanolamine (PE), it recruits Atg12-Atg5 to membranes by recognizing a noncanonical Atg8-interacting motif (AIM) within Atg12. Atg16 crosslinks Atg8-PE-Atg12-Atg5 complexes to form a continuous 2-dimensional membrane scaffold with meshwork-like architecture. Apparently, scaffold formation is required to generate productive autophagosomes and to deliver autophagic cargo to the vacuole in vivo.

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Sprache(n): eng - English
 Datum: 2014-07
 Publikationsstatus: Erschienen
 Seiten: 3
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000338946000017
DOI: 10.4161/auto.28980
 Art des Abschluß: -

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Titel: AUTOPHAGY
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA : LANDES BIOSCIENCE
Seiten: - Band / Heft: 10 (7) Artikelnummer: - Start- / Endseite: 1343 - 1345 Identifikator: ISSN: 1554-8627