English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Scaffolding the expansion of autophagosomes

Kaufmann, A., & Wollert, T. (2014). Scaffolding the expansion of autophagosomes. AUTOPHAGY, 10(7), 1343-1345. doi:10.4161/auto.28980.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Kaufmann, Anna1, Author              
Wollert, Thomas1, Author              
Affiliations:
1Wollert, Thomas / Molecular Membrane and Organelle Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565175              

Content

show
hide
Free keywords: macroautophagy; Atg8; conjugation; reconstitution; membrane scaffold;
 Abstract: The conjugation of the small ubiquitin (Ub)-like protein Atg8 to autophagic membranes is a key step during the expansion of phagophores. This reaction is driven by 2 interconnected Ub-like conjugation systems. The second system conjugates the Ub-like protein Atg12 to Atg5. The resulting conjugate catalyzes the covalent attachment of Atg8 to membranes. Atg12-Atg5, however, constitutively associates with the functionally less well-characterized coiled-coil protein Atg16. By reconstituting the conjugation of Atg8 to membranes in vitro, we showed that after Atg8 has been attached to phosphatidylethanolamine (PE), it recruits Atg12-Atg5 to membranes by recognizing a noncanonical Atg8-interacting motif (AIM) within Atg12. Atg16 crosslinks Atg8-PE-Atg12-Atg5 complexes to form a continuous 2-dimensional membrane scaffold with meshwork-like architecture. Apparently, scaffold formation is required to generate productive autophagosomes and to deliver autophagic cargo to the vacuole in vivo.

Details

show
hide
Language(s): eng - English
 Dates: 2014-07
 Publication Status: Published in print
 Pages: 3
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000338946000017
DOI: 10.4161/auto.28980
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: AUTOPHAGY
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA : LANDES BIOSCIENCE
Pages: - Volume / Issue: 10 (7) Sequence Number: - Start / End Page: 1343 - 1345 Identifier: ISSN: 1554-8627