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  The structure of the Pan2-Pan3 core complex reveals cross-talk between deadenylase and pseudokinase

Schäfer, I. B., Rode, M., Bonneau, F., Schüssler, S., & Conti, E. (2014). The structure of the Pan2-Pan3 core complex reveals cross-talk between deadenylase and pseudokinase. NATURE STRUCTURAL & MOLECULAR BIOLOGY, 21(7), 591-598. doi:10.1038/nsmb.2834.

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 Creators:
Schäfer, Ingmar B.1, Author              
Rode, Michaela1, Author              
Bonneau, Fabien1, Author              
Schüssler, Steffen1, Author              
Conti, Elena1, Author              
Affiliations:
1Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              

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Free keywords: MESSENGER-RNA TURNOVER; YEAST CCR4-NOT COMPLEX; POLY(A) NUCLEASE; POLY(A)-BINDING PROTEIN; CRYSTAL-STRUCTURE; SACCHAROMYCES-CEREVISIAE; GW182 PROTEINS; SUBUNIT; MODULE; DECAY
 Abstract: Pan2-Pan3 is a conserved complex involved in the shortening of mRNA poly(A) tails, the initial step in eukaryotic mRNA turnover. We show that recombinant Saccharomyces cerevisiae Pan2-Pan3 can deadenylate RNAs in vitro without needing the poly(A)-binding protein Pab1. The crystal structure of an active similar to 200-kDa core complex reveals that Pan2 and Pan3 interact with an unusual 1:2 stoichiometry imparted by the asymmetric nature of the Pan3 homodimer. An extended region of Pan2 wraps around Pan3 and provides a major anchoring point for complex assembly. A Pan2 module formed by the pseudoubiquitin-hydrolase and RNase domains latches onto the Pan3 pseudokinase with intertwined interactions that orient the deadenylase active site toward the A-binding site of the interacting Pan3. The molecular architecture of Pan2-Pan3 suggests how the nuclease and its pseudokinase regulator act in synergy to promote deadenylation.

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Language(s): eng - English
 Dates: 2014-07
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000338689800006
DOI: 10.1038/nsmb.2834
 Degree: -

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Title: NATURE STRUCTURAL & MOLECULAR BIOLOGY
Source Genre: Journal
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Publ. Info: 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 21 (7) Sequence Number: - Start / End Page: 591 - 598 Identifier: ISSN: 1545-9993