English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Surgical procedures to study microglial motility in the brain and in the spinal cord by in vivo two-photon laser-scanning microscopy.

Cupido, A., Catalin, B., Steffens, H., & Kirchhoff, F. (2014). Surgical procedures to study microglial motility in the brain and in the spinal cord by in vivo two-photon laser-scanning microscopy. In L. Bakota, & R. Brandt (Eds.), Laser scanning microscopy and quantitative image analysis of neuronal tissue (pp. 37-50). New York, N.Y.: Humana Pr. doi:10.1007/978-1-4939-0381-8_2,.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0023-C189-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CDF3-7
Genre: Book Chapter

Files

show Files
hide Files
:
2051567.pdf (Publisher version), 384KB
 
File Permalink:
-
Name:
2051567.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Cupido, A., Author
Catalin, B., Author
Steffens, H.1, Author              
Kirchhoff, F., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

Content

show
hide
Free keywords: Microglia; In vivo two-photon imaging; Spinal cord; Brain - Surgery; Transgenic mice
 Abstract: Microglia are the innate immune cells of the central nervous system (CNS). As such, they contribute to neural inflammation in a large number of neurodegenerative diseases. The processes of microglia are continuously moving to survey the brain parenchyma. Depending on local signals, microglia can almost instantaneously change their morphology. Two-photon laser-scanning microscopy (2P-LSM) has become the method of choice to study microglia in vivo. Here, we will describe in detail surgical procedures to prepare transgenic mice with fluorescent protein expression for 2P imaging of microglia in the cortex and the spinal cord. In addition, we will give some advice how to optimize image quality.

Details

show
hide
Language(s): eng - English
 Dates: 20142014-05-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1007/978-1-4939-0381-8_2,
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Laser scanning microscopy and quantitative image analysis of neuronal tissue
Source Genre: Book
 Creator(s):
Bakota, L., Editor
Brandt, R., Editor
Affiliations:
-
Publ. Info: New York, N.Y. : Humana Pr.
Pages: XII, 307 Volume / Issue: - Sequence Number: - Start / End Page: 37 - 50 Identifier: ISBN: 978-1-4939-0381-8

Source 2

show
hide
Title: Neuromethods
Source Genre: Series
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 87 Sequence Number: - Start / End Page: - Identifier: -