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  Presequence recognition by the Tom40 channel contributes to precursor translocation into the mitochondrial matrix.

Melin, J., Schulz, C., Wrobel, L., Bernhard, O., Chacinska, A., Jahn, O., et al. (2014). Presequence recognition by the Tom40 channel contributes to precursor translocation into the mitochondrial matrix. Molecular and Cellular Biology, 34(18), 3473-3485. doi:10.1128/MCB.00433-14.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0023-DB38-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-BBEC-A
Genre: Journal Article

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Melin, J., Author
Schulz, C., Author
Wrobel, L., Author
Bernhard, O., Author
Chacinska, A., Author
Jahn, O., Author
Schmidt, B., Author
Rehling, P.1, Author              
Affiliations:
1Max Planck Fellow Peter Rehling, ou_1298545              

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 Abstract: More than 70% of mitochondrial proteins utilize N-terminal presequences as targeting signals. Presequence interactions with redundant cytosolic receptor domains of the translocase of the outer mitochondrial membrane (TOM) are well established. However, after the presequence enters the protein-conducting Tom40 channel, the recognition events that occur at the trans side leading up to the engagement of the presequence with inner membrane-bound receptors are less well defined. Using a photoaffinity-labeling approach with modified presequence peptides, we identified Tom40 as a presequence interactor of the TOM complex. Utilizing mass spectrometry, we mapped Tom40's presequence-interacting regions to both sides of the β-barrel. Analysis of a phosphorylation site within one of the presequence-interacting regions revealed altered translocation kinetics along the presequence pathway. Our analyses assess the relation between the identified presequence-binding region of Tom40 and the intermembrane space domain of Tom22. The identified presequence-interacting region of Tom40 is capable of functioning independently of the established trans-acting TOM presequence-binding domain during matrix import.

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Language(s): eng - English
 Dates: 2014-07-072014-09
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1128/MCB.00433-14
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Title: Molecular and Cellular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 34 (18) Sequence Number: - Start / End Page: 3473 - 3485 Identifier: -