English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Programmed –1 frameshifting by kinetic partitioning during impeded translocation.

Caliskan, N., Katunin, V. I., Belardinelli, R., Peske, F., & Rodnina, M. V. (2014). Programmed –1 frameshifting by kinetic partitioning during impeded translocation. Cell, 157(7), 1619-1631. doi:10.1016/j.cell.2014.04.041.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0023-DC71-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-DBC1-8
Genre: Journal Article

Files

show Files
hide Files
:
2058311.pdf (Publisher version), 3MB
Name:
2058311.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2058311_Suppl_1.pdf (Supplementary material), 121KB
Name:
2058311_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2058311_Suppl_2.pdf (Supplementary material), 3MB
Name:
2058311_Suppl_2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Caliskan, N.1, Author              
Katunin, V. I., Author
Belardinelli, R.1, Author              
Peske, F.1, Author              
Rodnina, M. V.1, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              

Content

show
hide
Free keywords: -
 Abstract: Programmed –1 ribosomal frameshifting (−1PRF) is an mRNA recoding event utilized by cells to enhance the information content of the genome and to regulate gene expression. The mechanism of –1PRF and its timing during translation elongation are unclear. Here, we identified the steps that govern –1PRF by following the stepwise movement of the ribosome through the frameshifting site of a model mRNA derived from the IBV 1a/1b gene in a reconstituted in vitro translation system from Escherichia coli. Frameshifting occurs at a late stage of translocation when the two tRNAs are bound to adjacent slippery sequence codons of the mRNA. The downstream pseudoknot in the mRNA impairs the closing movement of the 30S subunit head, the dissociation of EF-G, and the release of tRNA from the ribosome. The slippage of the ribosome into the –1 frame accelerates the completion of translocation, thereby further favoring translation in the new reading frame.

Details

show
hide
Language(s): eng - English
 Dates: 2014-06-19
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cell.2014.04.041
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 157 (7) Sequence Number: - Start / End Page: 1619 - 1631 Identifier: -