English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  The cytoplasmic PAS(C) domain of the sensor kinase DcuS of Escherichia coli: Role in signal transduction, dimer formation, and DctA interaction.

Monzel, C., Degreif-Dünnwald, P., Gröpper, C., Griesinger, C., & Unden, G. (2013). The cytoplasmic PAS(C) domain of the sensor kinase DcuS of Escherichia coli: Role in signal transduction, dimer formation, and DctA interaction. Microbiologypen, 2(6), 912-927. doi:10.1002/mbo3.127.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-02F0-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CC09-5
Genre: Journal Article

Files

show Files
hide Files
:
2065717.pdf (Publisher version), 2MB
Name:
2065717.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2065717_Suppl_1.pdf (Supplementary material), 231KB
Name:
2065717_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2065717_Suppl_2.pdf (Supplementary material), 246KB
Name:
2065717_Suppl_2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2065717_Suppl_3.pdf (Supplementary material), 280KB
Name:
2065717_Suppl_3.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2065717_Suppl_4.pdf (Supplementary material), 150KB
Name:
2065717_Suppl_4.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2065717_Suppl_5.doc (Supplementary material), 974KB
Name:
2065717_Suppl_5.doc
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/msword / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Monzel, C., Author
Degreif-Dünnwald, P., Author
Gröpper, C., Author
Griesinger, C.1, Author              
Unden, G., Author
Affiliations:
1Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

Content

show
hide
Free keywords: DctA; DcuS sensor kinase; fumarate; PAS domain; signal transduction
 Abstract: The cytoplasmic PAS(C) domain of the fumarate responsive sensor kinase DcuS of Escherichia coli links the transmembrane to the kinase domain. PAS(C) is also required for interaction with the transporter DctA serving as a cosensor of DcuS. Earlier studies suggested that PAS(C) functions as a hinge and transmits the signal to the kinase. Reorganizing the PAS(C) dimer interaction and, independently, removal of DctA, converts DcuS to the constitutive ON state (active without fumarate stimulation). ON mutants were categorized with respect to these two biophysical interactions and the functional state of DcuS: type I-ON mutations grossly reorganize the homodimer, and decrease interaction with DctA. Type IIA-ON mutations create the ON state without grossly reorganizing the homodimer, whereas interaction with DctA is decreased. The type IIB-ON mutations were neither in PAS(C)/PAS(C), nor in DctA/DcuS interaction affected, similar to fumarate activated wild-typic DcuS. OFF mutations never affected dimer stability. The ON mutations provide novel mechanistic insight: PAS(C) dimerization is essential to silence the kinase. Reorganizing the homodimer and its interaction with DctA activate the kinase. The study suggests a novel ON homo-dimer conformation (type IIB) and an OFF conformation for PAS(C). Type IIB-ON corresponds to the fumarate induced wild-type conformation, representing an interesting target for structural biology.

Details

show
hide
Language(s): eng - English
 Dates: 2013-09-092013-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/mbo3.127
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Microbiologypen
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 2 (6) Sequence Number: - Start / End Page: 912 - 927 Identifier: -