English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Histones to the cytosol: Exportin 7 is essential for normal terminal erythroid nuclear maturation.

Hattangadi, S. M., Martinez-Morilla, S., Patterson, H. C., Shi, J., Burke, K., Avila-Figueroa, A., et al. (2014). Histones to the cytosol: Exportin 7 is essential for normal terminal erythroid nuclear maturation. Blood, 124(12), 1931-1940. doi:10.1182/blood-2013-11-537761.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-0755-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CC0D-E
Genre: Journal Article

Files

show Files
hide Files
:
2065956_Suppl_1.pdf (Supplementary material), 3MB
Name:
2065956_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2065956_Suppl_2.xls (Supplementary material), 47KB
Name:
2065956_Suppl_2.xls
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.ms-excel / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Hattangadi, S. M., Author
Martinez-Morilla, S., Author
Patterson, H. C., Author
Shi, J., Author
Burke, K., Author
Avila-Figueroa, A., Author
Venkatesan, S., Author
Wang, J., Author
Paulsen, K.1, Author              
Görlich, D.1, Author              
Murata-Hori, M., Author
Lodish, H. F., Author
Affiliations:
1Department of Cellular Logistics, Max Planck Institute for biophysical chemistry, Max Planck Society, ou_578574              

Content

show
hide
Free keywords: -
 Abstract: Global nuclear condensation, culminating in enucleation during terminal erythropoiesis, is poorly understood. Proteomic examination of extruded erythroid nuclei from fetal liver revealed a striking depletion of most nuclear proteins, suggesting that nuclear protein export had occurred. Expression of the nuclear export protein, Exportin 7 (Xpo7), is highly erythroid-specific, induced during erythropoiesis, and abundant in very late erythroblasts. Knockdown of Xpo7 in primary mouse fetal liver erythroblasts resulted in severe inhibition of chromatin condensation and enucleation but otherwise had little effect on erythroid differentiation, including hemoglobin accumulation. Nuclei in Xpo7-knockdown cells were larger and less dense than normal and accumulated most nuclear proteins as measured by mass spectrometry. Strikingly, many DNA binding proteins such as histones H2A and H3 were found to have migrated into the cytoplasm of normal late erythroblasts prior to and during enucleation, but not in Xpo7-knockdown cells. Thus, terminal erythroid maturation involves migration of histones into the cytoplasm via a process likely facilitated by Xpo7.

Details

show
hide
Language(s): eng - English
 Dates: 2014-09-18
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1182/blood-2013-11-537761
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Blood
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 124 (12) Sequence Number: - Start / End Page: 1931 - 1940 Identifier: -