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  Time-resolved protein nanocrystallography using an X-ray free-electron laser

Aquila, A., Hunter, M. S., Doak, R. B., Kirian, R. A., Fromme, P., White, T. A., et al. (2012). Time-resolved protein nanocrystallography using an X-ray free-electron laser. Optics Express, 20(3), 2706-2716. doi:10.1364/OE.20.002706.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-1055-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-1056-E
Genre: Journal Article
Alternative Title : Time-resolved protein nanocrystallography using an X-ray free-electron laser

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http://dx.doi.org/10.1364/OE.20.002706 (Any fulltext)
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 Creators:
Aquila, Andrew, Author
Hunter, Mark S., Author
Doak, R. Bruce1, Author              
Kirian, Richard A., Author
Fromme, Petra, Author
White, Thomas A., Author
Andreasson, Jakob, Author
Arnlund, David, Author
Bajt, Saša, Author
Barends, Thomas1, Author              
Barthelmess, Miriam, Author
Bogan, Michael J., Author
Bostedt, Christoph, Author
Bottin, Hervé, Author
Bozek, John D., Author
Caleman, Carl, Author
Coppola, Nicola, Author
Davidsson, Jan, Author
DePonte, Daniel P., Author
Elser, Veit, Author
Epp, Sascha W., AuthorErk, Benjamin1, Author              Fleckenstein, Holger, AuthorFoucar, Lutz1, Author              Frank, Matthias, AuthorFromme, Raimund, AuthorGraafsma, Heinz, AuthorGrotjohann, Ingo, AuthorGumprecht, Lars, AuthorHajdu, Janos, AuthorHampton, Christina Y., AuthorHartmann, Andreas, AuthorHartmann, Robert, AuthorHau−Riege, Stefan P., AuthorHauser, Günter, AuthorHirsemann, Helmut, AuthorHoll, Peter, AuthorHolton, James M., AuthorHömke, André, AuthorJohansson, Linda, AuthorKimmel, Nils, AuthorKassemeyer, Stephan1, Author              Krasniqi, Faton1, Author              Kühnel, Kai−Uwe, AuthorLiang, Mengning, AuthorLomb, Lukas1, Author              Malmerberg, Erik, AuthorMarchesini, Stefano, AuthorMartin, Andrew V., AuthorMaia, Filipe R.N.C., AuthorMesserschmidt, Marc, AuthorNass, Karol1, Author              Reich, Christian, AuthorNeutze, Richard, AuthorRolles, Daniel1, Author              Rudek, Benedikt1, Author              Rudenko, Artem, AuthorSchlichting, Ilme1, Author              Schmidt, Carlo, AuthorSchmidt, Kevin E., AuthorSchulz, Joachim, AuthorSeibert, M. Marvin, AuthorShoeman, Robert L.1, Author              Sierra, Raymond G., AuthorSoltau, Heike, AuthorStarodub, Dmitri, AuthorStellato, Francesco, AuthorStern, Stephan, AuthorStrüder, Lothar, AuthorTimneanu, Nicusor, AuthorUllrich, Joachim, AuthorWang, Xiaoyu, AuthorWilliams, Garth J., AuthorWeidenspointner, Georg, AuthorWeierstall, Uwe, AuthorWunderer, Cornelia B., AuthorBarty, Anton, AuthorSpence, John C. H., AuthorChapman, Henry N., Author more..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: We demonstrate the use of an X−ray free electron laser synchronized with an optical pump laser to obtain X−ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light−induced changes of Photosystem I−Ferredoxin co−crystals were observed at time delays of 5 to 10 μs after excitation. The result correlates with the microsecond kinetics of electron transfer from Photosystem I to ferredoxin. The undocking process that follows the electron transfer leads to large rearrangements in the crystals that will terminally lead to the disintegration of the crystals. We describe the experimental setup and obtain the first timeresolved femtosecond serial X−ray crystallography results from an irreversible photo−chemical reaction at the Linac Coherent Light Source. This technique opens the door to time−resolved structural studies of reaction dynamics in biological systems

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Language(s): eng - English
 Dates: 2011-11-112011-12-182012-01-30
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Optics Express
Source Genre: Journal
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Publ. Info: Washington, DC : Optical Society of America
Pages: - Volume / Issue: 20 (3) Sequence Number: - Start / End Page: 2706 - 2716 Identifier: ISSN: 1094-4087
CoNE: https://pure.mpg.de/cone/journals/resource/954925609918