Time-resolved protein nanocrystallography using an X-ray free-electron laser

Aquila, Andrew; Hunter, Mark S.; Doak, R. Bruce; Kirian, Richard A.; Fromme, Petra; White, Thomas A.; Andreasson, Jakob; Arnlund, David; Bajt, Saša; Barends, Thomas; Barthelmess, Miriam; Bogan, Michael J.; Bostedt, Christoph; Bottin, Hervé; Bozek, John D.; Caleman, Carl; Coppola, Nicola; Davidsson, Jan; DePonte, Daniel P.; Elser, Veit; Epp, Sascha W.; Erk, Benjamin; Fleckenstein, Holger; Foucar, Lutz; Frank, Matthias; Fromme, Raimund; Graafsma, Heinz; Grotjohann, Ingo; Gumprecht, Lars; Hajdu, Janos; Hampton, Christina Y.; Hartmann, Andreas; Hartmann, Robert; Hau−Riege, Stefan P.; Hauser, Günter; Hirsemann, Helmut; Holl, Peter; Holton, James M.; Hömke, André; Johansson, Linda; Kimmel, Nils; Kassemeyer, Stephan; Krasniqi, Faton; Kühnel, Kai−Uwe; Liang, Mengning; Lomb, Lukas; Malmerberg, Erik; Marchesini, Stefano; Martin, Andrew V.; Maia, Filipe R.N.C.; Messerschmidt, Marc; Nass, Karol; Reich, Christian; Neutze, Richard; Rolles, Daniel; Rudek, Benedikt; Rudenko, Artem; Schlichting, Ilme; Schmidt, Carlo; Schmidt, Kevin E.; Schulz, Joachim; Seibert, M. Marvin; Shoeman, Robert L.; Sierra, Raymond G.; Soltau, Heike; Starodub, Dmitri; Stellato, Francesco; Stern, Stephan; Strüder, Lothar; Timneanu, Nicusor; Ullrich, Joachim; Wang, Xiaoyu; Williams, Garth J.; Weidenspointner, Georg; Weierstall, Uwe; Wunderer, Cornelia B.; Barty, Anton; Spence, John C. H.; Chapman, Henry N.

doi:10.1364/OE.20.002706

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Time-resolved protein nanocrystallography using an X-ray free-electron laser

Aquila, A., Hunter, M. S., Doak, R. B., Kirian, R. A., Fromme, P., White, T. A., et al. (2012). Time-resolved protein nanocrystallography using an X-ray free-electron laser. Optics Express, 20(3), 2706-2716. doi:10.1364/OE.20.002706.

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Alternativer Titel : Time-resolved protein nanocrystallography using an X-ray free-electron laser

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Aquila, Andrew, Autor
Hunter, Mark S., Autor
Doak, R. Bruce¹, Autor
Kirian, Richard A., Autor
Fromme, Petra, Autor
White, Thomas A., Autor
Andreasson, Jakob, Autor
Arnlund, David, Autor
Bajt, Saša, Autor
Barends, Thomas¹, Autor
Barthelmess, Miriam, Autor
Bogan, Michael J., Autor
Bostedt, Christoph, Autor
Bottin, Hervé, Autor
Bozek, John D., Autor
Caleman, Carl, Autor
Coppola, Nicola, Autor
Davidsson, Jan, Autor
DePonte, Daniel P., Autor
Elser, Veit, Autor
mehr..

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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700

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Zusammenfassung: We demonstrate the use of an X−ray free electron laser synchronized with an optical pump laser to obtain X−ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light−induced changes of Photosystem I−Ferredoxin co−crystals were observed at time delays of 5 to 10 μs after excitation. The result correlates with the microsecond kinetics of electron transfer from Photosystem I to ferredoxin. The undocking process that follows the electron transfer leads to large rearrangements in the crystals that will terminally lead to the disintegration of the crystals. We describe the experimental setup and obtain the first timeresolved femtosecond serial X−ray crystallography results from an irreversible photo−chemical reaction at the Linac Coherent Light Source. This technique opens the door to time−resolved structural studies of reaction dynamics in biological systems

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Sprache(n): eng - English

Datum: Eingereicht: 2011-11-11Angenommen: 2011-12-18Erschienen: 2012-01-30

Publikationsstatus: Erschienen

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Art der Begutachtung: Expertenbegutachtung

Identifikatoren: eDoc: 664426
DOI: 10.1364/OE.20.002706
URI: http://www.ncbi.nlm.nih.gov/pubmed/22330507
URI: http://www.opticsinfobase.org/oe/viewmedia.cfm?uri=oe-20-3-2706&seq=0&html=true
URI: http://www.opticsinfobase.org/oe/viewmedia.cfm?uri=oe-20-3-2706&seq=0
Anderer: 7760

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Titel: Optics Express

Genre der Quelle: Zeitschrift

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Ort, Verlag, Ausgabe: Washington, DC : Optical Society of America

Seiten: - Band / Heft: 20 (3) Artikelnummer: - Start- / Endseite: 2706 - 2716 Identifikator: ISSN: 1094-4087
CoNE: https://pure.mpg.de/cone/journals/resource/954925609918

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