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  High-accuracy neurite reconstruction for high-throughput neuroanatomy

Helmstaedter, M., Briggman, K., & Denk, W. (2011). High-accuracy neurite reconstruction for high-throughput neuroanatomy. Nature Neuroscience, 14(8), 1081-1088. doi:10.1038/nn.2868.

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Alternative Title : High-accuracy neurite reconstruction for high-throughput neuroanatomy

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Helmstaedter, Moritz1, 2, Author           
Briggman, Kevin1, Author           
Denk, Winfried1, Author           
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1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: Neuroanatomic analysis depends on the reconstruction of complete cell shapes. High-throughput reconstruction of neural circuits, or connectomics, using volume electron microscopy requires dense staining of all cells, which leads even experts to make annotation errors. Currently, reconstruction speed rather than acquisition speed limits the determination of neural wiring diagrams. We developed a method for fast and reliable reconstruction of densely labeled data sets. Our approach, based on manually skeletonizing each neurite redundantly (multiple times) with a visualization-annotation software tool called KNOSSOS, is ˜50-fold faster than volume labeling. Errors are detected and eliminated by a redundant-skeleton consensus procedure (RESCOP), which uses a statistical model of how true neurite connectivity is transformed into annotation decisions. RESCOP also estimates the reliability of consensus skeletons. Focused reannotation of difficult locations promises a rather steep increase of reliability as a function of the average skeleton redundancy and thus the nearly error-free analysis of large neuroanatomical datasets

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Language(s): eng - English
 Dates: 2011-02-282011-05-232011-07-102011-08-01
 Publication Status: Published in print
 Pages: 8
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 664481
DOI: 10.1038/nn.2868
Other: 7694
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Title: Nature Neuroscience
  Other : Nat. Neurosci.
Source Genre: Journal
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Publ. Info: New York, NY : Nature America Inc.
Pages: - Volume / Issue: 14 (8) Sequence Number: - Start / End Page: 1081 - 1088 Identifier: ISSN: 1097-6256
CoNE: https://pure.mpg.de/cone/journals/resource/954925610931