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  Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals

Kirian, R. A., White, T. A., Holton, J. M., Chapman, H. N., Fromme, P., Barty, A., et al. (2011). Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals. Acta Crystallographica A, 67(2), 131-140. doi:10.1107/S0108767310050981.

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Genre: Journal Article
Alternative Title : Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals

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 Creators:
Kirian, Richard A., Author
White, Thomas A., Author
Holton, James M., Author
Chapman, Henry N., Author
Fromme, Petra, Author
Barty, Anton, Author
Lomb, Lukas1, Author           
Aquila, Andrew, Author
Maia, Filipe R. N. C., Author
Martin, Andrew V., Author
Fromme, Raimund, Author
Wang, Xiaoyu, Author
Hunter, Mark S., Author
Schmidt, Kevin E., Author
Spence, John C. H., Author
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: nanocrystals; femtosecond diffraction; free−electron lasers; Monte Carlo methods; protein microdiffraction
 Abstract: A complete set of structure factors has been extracted from hundreds of thousands of femtosecond single−shot X−ray microdiffraction patterns taken from randomly oriented nanocrystals. The method of Monte Carlo integration over crystallite size and orientation was applied to experimental data from Photosystem I nanocrystals. This arrives at structure factors from many partial reflections without prior knowledge of the particle−size distribution. The data were collected at the Linac Coherent Light Source (the first hard−X−ray laser user facility), to which was fitted a hydrated protein nanocrystal injector jet, according to the method of serial crystallography. The data are single `still' diffraction snapshots, each from a different nanocrystal with sizes ranging between 100 nm and 2 µm, so the angular width of Bragg peaks was dominated by crystal−size effects. These results were compared with single−crystal data recorded from large crystals of Photosystem I at the Advanced Light Source and the quality of the data was found to be similar. The implications for improving the efficiency of data collection by allowing the use of very small crystals, for radiation−damage reduction and for time−resolved diffraction studies at room temperature are discussed

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Language(s): eng - English
 Dates: 2010-08-252010-12-052011-03-01
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 664436
DOI: 10.1107/S0108767310050981
Other: 7753
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Title: Acta Crystallographica A
Source Genre: Journal
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Publ. Info: Oxford, England : Blackwell Publishing on behalf of the International Union of Crystallography
Pages: - Volume / Issue: 67 (2) Sequence Number: - Start / End Page: 131 - 140 Identifier: ISSN: 0108-7673
CoNE: https://pure.mpg.de/cone/journals/resource/954927679544