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  Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Lomb, L., Barends, T., Kassemeyer, S., Aquila, A., Epp, S. W., Erk, B., et al. (2011). Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser. Physical Review B, 84(21): e214111, pp. 1-6. doi:10.1103/PhysRevB.84.214111.

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Alternative Title : Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

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PhysRevB_84_2011_ 0214111e.pdf (Any fulltext), 571KB
 
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 Creators:
Lomb, Lukas1, Author           
Barends, Thomas1, Author           
Kassemeyer, Stephan1, Author           
Aquila, Andrew, Author
Epp, Sascha W., Author
Erk, Benjamin1, Author           
Foucar, Lutz1, Author           
Hartmann, Robert, Author
Rudek, Benedikt1, Author           
Rolles, Daniel1, Author           
Rudenko, Artem, Author
Shoeman, Robert L.1, Author           
Andreasson, Jakob, Author
Bajt, Saša, Author
Barthelmess, Miriam, Author
Barty, Anton, Author
Bogan, Michael J., Author
Bostedt, Christoph, Author
Bozek, John D., Author
Caleman, Carl, Author
Coffee, Ryan, AuthorCoppola, Nicola, AuthorDePonte, Daniel P., AuthorDoak, R. Bruce1, Author           Ekeberg, Tomas, AuthorFleckenstein, Holger, AuthorFromme, Petra, AuthorGebhardt, Maike1, Author           Graafsma, Heinz, AuthorGumprecht, Lars, AuthorHampton, Christina Y., AuthorHartmann, Andreas, AuthorHauser, Günter, AuthorHirsemann, Helmut, AuthorHoll, Peter, AuthorHolton1, James M., AuthorHunter, Mark S., AuthorKabsch, Wolfgang1, Author           Kimmel, Nils, AuthorKirian, Richard A., AuthorLiang, Mengning, AuthorMaia, Filipe R. N. C., AuthorMeinhart, Anton1, Author           Marchesini, Stefano, AuthorMartin, Andrew V., AuthorNass, Karol1, Author           Reich, Christian, AuthorSchulz, Joachim, AuthorSeibert, M. Marvin, AuthorSierra, Raymond G., AuthorSoltau, Heike, AuthorSpence, John C. H., AuthorSteinbrener, Jan1, Author           Stellato, Francesco, AuthorStern, Stephan, AuthorTimneanu, Nicusor, AuthorWang, Xiaoyu, AuthorWeidenspointner, Georg, AuthorWeierstall, Uwe, AuthorWhite, Thomas A., AuthorWunderer, Cornelia B., AuthorChapman, Henry N., AuthorUllrich, Joachim, AuthorStrüder, Lothar, AuthorSchlichting, Ilme1, Author            more..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: X−ray free−electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects

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Language(s): eng - English
 Dates: 2011-07-232011-12-20
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 664467
DOI: 10.1103/PhysRevB.84.214111
Other: 7734
 Degree: -

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Title: Physical Review B
  Other : Phys. Rev. B
Source Genre: Journal
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Publ. Info: Woodbury, NY : American Physical Society
Pages: - Volume / Issue: 84 (21) Sequence Number: e214111 Start / End Page: 1 - 6 Identifier: ISSN: 1098-0121
CoNE: https://pure.mpg.de/cone/journals/resource/954925225008