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  In vivo protein crystallization opens new routes in structural biology

Koopmann, R., Cupelli, K., Redecke, L., Nass, K., DePonte, D. P., White, T. A., et al. (2012). In vivo protein crystallization opens new routes in structural biology. Nature methods, 9(3), 259-262. doi:10.1038/nmeth.1859.

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Alternative Title : In vivo protein crystallization opens new routes in structural biology

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 Creators:
Koopmann, Rudolf, Author
Cupelli, Karolina, Author
Redecke, Lars, Author
Nass, Karol1, Author              
DePonte, Daniel P., Author
White, Thomas A., Author
Stellato, Francesco, Author
Rehders, Dirk, Author
Liang, Mengning, Author
Andreasson, Jakob, Author
Aquila, Andrew, Author
Bajt, Saša, Author
Barthelmess, Miriam, Author
Barty, Anton, Author
Bogan, Michael J., Author
Bostedt, Christoph, Author
Boutet, Sébastien, Author
Bozek, John D., Author
Caleman, Carl, Author
Coppola, Nicola, Author
Davidsson, Jan, AuthorDoak, R. Bruce1, Author              Ekeberg, Tomas, AuthorEpp, Sascha W., AuthorErk, Benjamin1, Author              Fleckenstein, Holger, AuthorFoucar, Lutz1, Author              Graafsma, Heinz, AuthorGumprecht, Lars, AuthorHajdu, Janos, AuthorHampton, Christina. Y, AuthorHartmann, Andreas, AuthorHartmann, Robert, AuthorHauser, Günter, AuthorHirsemann, Helmut, AuthorHoll, Peter, AuthorHunter, Mark S., AuthorKassemeyer, Stephan1, Author              Kirian, Richard A., AuthorLomb, Lukas1, Author              Maia, Filipe R. N. C., AuthorKimmel, Nils, AuthorMartin, Andrew V., AuthorMesserschmidt, Marc, AuthorReich, Christian, AuthorRolles, Daniel1, Author              Rudek, Benedikt1, Author              Rudenko, Artem, AuthorSchlichting, Ilme1, Author              Schulz, Joachim, AuthorSeibert, M. Marvin, AuthorShoeman, Robert L.1, Author              Sierra, Raymond G., AuthorSoltau, Heike, AuthorStern, Stephan, AuthorStrüder, Lothar, AuthorTimneanu, Nicusor, AuthorUllrich, Joachim, AuthorWang, Xiaoyu, AuthorWeidenspointner, Georg, AuthorWeierstall, Uwe, AuthorWilliams, Garth J., AuthorWunderer, Cornelia B., AuthorFromme, Petra, AuthorSpence, John C. H., AuthorStehle, Thilo, AuthorChapman, Henry N., AuthorBetzel, Christian, AuthorDuszenko, Michael, Author more..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X−ray crystallographic analysis. We prepared nano−sized in vivo−grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free−electron laser−based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology

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Language(s): eng - English
 Dates: 2011-05-022011-12-212012-01-29
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 9 (3) Sequence Number: - Start / End Page: 259 - 262 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556