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  Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo

Mittmann, W., Wallace, D. J., Czubayko, U., Herb, J. T., Schaefer, A. T., Looger, L. L., et al. (2011). Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo. Nature Neuroscience, 14(8), 1089-1093. doi:10.1038/nn.2879.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-1E21-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-1E22-3
Genre: Journal Article
Alternative Title : Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo

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 Creators:
Mittmann, Wolfgang1, Author              
Wallace, Damian J, Author
Czubayko, Uwe, Author
Herb, Jan T., Author
Schaefer, Andreas T.2, Author              
Looger, Loren L., Author
Denk, Winfried1, Author              
Kerr, Jason N D, Author
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: Multiphoton imaging (MPI) is widely used for recording activity simultaneously from many neurons in superficial cortical layers in vivo. We combined regenerative amplification multiphoton microscopy (RAMM) with genetically encoded calcium indicators to extend MPI of neuronal population activity into layer 5 (L5) of adult mouse somatosensory cortex. We found that this approach could be used to record and quantify spontaneous and sensory−evoked activity in populations of L5 neuronal somata located as much as 800 μm below the pia. In addition, we found that RAMM could be used to simultaneously image activity from large (80) populations of apical dendrites and follow these dendrites down to their somata of origin

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Language(s): eng - English
 Dates: 2010-12-022011-05-162011-07-102011-08-01
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 664456
DOI: 10.1038/nn.2879
Other: 7722
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Title: Nature Neuroscience
  Other : Nat. Neurosci.
Source Genre: Journal
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Publ. Info: New York, NY : Nature America Inc.
Pages: - Volume / Issue: 14 (8) Sequence Number: - Start / End Page: 1089 - 1093 Identifier: ISSN: 1097-6256
CoNE: https://pure.mpg.de/cone/journals/resource/954925610931