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  An anti-settling sample delivery instrument for serial femtosecond crystallography

Lomb, L., Steinbrener, J., Bari, S., Beisel, D., Berndt, D., Kieser, C., et al. (2012). An anti-settling sample delivery instrument for serial femtosecond crystallography. Journal of Applied Crystallography, 45(4), 674-678. doi:10.1107/S0021889812024557.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-1E28-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-1E29-6
Genre: Journal Article
Alternative Title : An anti-settling sample delivery instrument for serial femtosecond crystallography

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JApplCryst_45_2012_674.pdf (Any fulltext), 441KB
 
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 Creators:
Lomb, Lukas1, Author              
Steinbrener, Jan1, Author              
Bari, Sadia, Author
Beisel, Daniel2, Author              
Berndt, Daniel2, Author              
Kieser, Christian1, Author              
Lukat, Martin2, Author              
Neef, Niklas2, Author              
Shoeman, Robert L.1, Author              
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: serial femtosecond crystallography; X−ray free−electron lasers; macromolecular crystallography; anti−settling; sample delivery instruments
 Abstract: Serial femtosecond crystallography (SFX) using X−ray free−electron laser (FEL) sources has the potential to determine the structures of macromolecules beyond the limitation of radiation damage and without the need for crystals of sufficient size for conventional crystallography. In SFX, a liquid microjet is used to inject randomly oriented crystals suspended in their storage solution into the FEL beam. Settling of crystals in the reservoir prior to the injection has been found to complicate the data collection. This article details the development of an antisettling sample delivery instrument based on a rotating syringe pump, capable of producing flow rates and liquid pressures necessary for the operation of the injector. The device has been used successfully with crystals of different proteins, with crystal sizes smaller than 20 mm. Even after hours of continuous operation, no significant impairment of the experiments due to sample settling was observed. This article describes the working principle of the instrument and sets it in context with regard to the experimental conditions used for SFX. Hit rates for longer measuring periods are compared with and without the instrument operating. Two versions of the instrument have been developed, which both deliver sample at a constant flow rate but which differ in their minimum liquid flow rates and maximum pressures

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Language(s): eng - English
 Dates: 2012-02-282012-05-292012-07-042012-08-01
 Publication Status: Published in print
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 Rev. Type: Peer
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Title: Journal of Applied Crystallography
Source Genre: Journal
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Publ. Info: Oxford, England : Blackwell Publishing on behalf of the International Union of Crystallography
Pages: - Volume / Issue: 45 (4) Sequence Number: - Start / End Page: 674 - 678 Identifier: ISSN: 0021-8898
CoNE: https://pure.mpg.de/cone/journals/resource/954925410812