English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Single mimivirus particles intercepted and imaged with an X-ray laser

Seibert, M. M., Ekeberg, T., Maia, F. R. N. C., Svenda, M., Andreasson, J., Odic, D., et al. (2011). Single mimivirus particles intercepted and imaged with an X-ray laser. Nature, 470(7332), 78-81. doi:10.1038/nature09748.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-1F01-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-1F02-3
Genre: Journal Article
Alternative Title : Single mimivirus particles intercepted and imaged with an X-ray laser

Files

show Files
hide Files
:
Nature_470_2011_78.pdf (Any fulltext), 849KB
 
File Permalink:
-
Name:
Nature_470_2011_78.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Locator:
http://dx.doi.org/10.1038/nature09748 (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Seibert, M. Marvin, Author
Ekeberg, Tomas, Author
Maia, Filipe R. N. C., Author
Svenda, Martin, Author
Andreasson, Jakob, Author
Odic, Duško, Author
Jönsson, Olof, Author
Iwan, Bianca, Author
Rocker, Andrea1, Author              
Westphal, Daniel, Author
Hantke, Max, Author
DePonte, Daniel P., Author
Barty, Anton, Author
Schulz, Joachim, Author
Gumprecht, Lars, Author
Coppola, Nicola, Author
Aquila, Andrew, Author
Liang, Mengning, Author
White, Thomas A., Author
Martin, Andrew V., Author
Caleman, Carl, AuthorStern, Stephan, AuthorAbergel, Chantal, AuthorSeltzer, Virginie, AuthorClaverie, Jean−Michel, AuthorBostedt, Christoph, AuthorBozek, John D., AuthorBoutet, Sébastien, AuthorMiahnahri, A. Alan, AuthorMesserschmidt, Marc, AuthorKrzywinski, Jacek, AuthorWilliams, Garth J., AuthorHodgson, Keith O., AuthorBogan, Michael J., AuthorHampton, Christina Y., AuthorSierra, Raymond G., AuthorStarodub, Dmitri, AuthorAndersson, Inger, AuthorBajt, Saša, AuthorBarthelmess, Miriam, AuthorSpence, John C. H., AuthorFromme, Petra, AuthorWeierstall, Uwe, AuthorKirian, Richard A., AuthorHunter, Mark S., AuthorDoak, R. Bruce1, Author              Marchesini, Stefano, AuthorHau−Riege, Stefan P., AuthorFrank, Matthias, AuthorShoeman, Robert L.1, Author              Lomb, Lukas1, Author              Epp, Sascha W., AuthorHartmann, Robert, AuthorRolles, Daniel1, Author              Rudenko, Artem, AuthorSchmidt, Carlo, AuthorFoucar, Lutz1, Author              Kimmel, Nils, AuthorHoll, Peter, AuthorRudek, Benedikt1, Author              Erk, Benjamin1, Author              Hömke, André, AuthorReich, Christian, AuthorPietschner, Daniel, AuthorWeidenspointner, Georg, AuthorStrüder, Lothar, AuthorHauser, Günter, AuthorGorke, Hubert, AuthorUllrich, Joachim, AuthorSchlichting, Ilme1, Author              Herrmann, Sven, AuthorSchaller, Gerhard, AuthorSchopper, Florian, AuthorSoltau, Heike, AuthorKühnel, Kai−Uwe, AuthorAndritschke, Robert, AuthorSchroeter, Claus Dieter, AuthorKrasniqi, Faton1, Author              Bott, Mario1, Author              Schorb, Sebastian, AuthorRupp, Daniela, AuthorAdolph, Marcus, AuthorGorkhover, Tais, AuthorHirsemann, Helmut, AuthorPotdevin, Guillaume, AuthorGraafsma, Heinz, AuthorNilsson, Björn, AuthorChapman, Henry N., AuthorHajdu, Janos, Author more..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Content

show
hide
Free keywords: -
 Abstract: X−ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X−ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non−crystalline objects permits oversampling and direct phase retrieval. Here we show that high−quality diffraction data can be obtained with a single X−ray pulse from a non−crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard−X−ray free−electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000?K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32−nm full−period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies

Details

show
hide
Language(s): eng - English
 Dates: 2010-07-162010-12-092011-02-022011-02-03
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 470 (7332) Sequence Number: - Start / End Page: 78 - 81 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238