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  Single-particle structure determination by correlations of snapshot X-ray diffraction patterns

Starodub, D., Aquila, A., Bajt, S., Barthelmess, M., Barty, A., Bostedt, C., et al. (2012). Single-particle structure determination by correlations of snapshot X-ray diffraction patterns. Nature Communications, 3: 1276, pp. 1-7. doi:10.1038/ncomms2288.

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Alternative Title : Single-particle structure determination by correlations of snapshot X-ray diffraction patterns

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 Creators:
Starodub, Dmitri, Author
Aquila, Andrew, Author
Bajt, Saša, Author
Barthelmess, Miriam, Author
Barty, Anton, Author
Bostedt, Christoph, Author
Bozek, John D., Author
Coppola, Nicola, Author
Doak, R. Bruce1, Author              
Epp, Sascha W., Author
Erk, Benjamin1, Author              
Foucar, Lutz1, Author              
Gumprecht, Lars, Author
Hampton, Christina Y., Author
Hartmann, Andreas, Author
Hartmann, Robert, Author
Holl, Peter, Author
Kassemeyer, Stephan1, Author              
Kimmel, Nils, Author
Laksmono, Hartawan, Author
Liang, Mengning, AuthorLoh, Ne-Te Duane, AuthorLomb, Lukas1, Author              Martin, Andrew V., AuthorNass, Karol1, Author              Reich, Christian, AuthorRolles, Daniel1, Author              Rudek, Benedikt1, Author              Rudenko, Artem, AuthorSchulz, Joachim, AuthorShoeman, Robert L.1, Author              Sierra, Raymond G., AuthorSoltau, Heike, AuthorSteinbrener, Jan1, Author              Stellato, Francesco, AuthorStern, Stephan, AuthorWeidenspointner, Georg, AuthorFrank, Matthias, AuthorUllrich, Joachim, AuthorStrüder, Lothar, AuthorSchlichting, Ilme1, Author              Chapman, Henry N., AuthorSpence, John C. H., AuthorBogan, Michael J., Author more..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Diffractive imaging with free−electron lasers allows structure determination from ensembles of weakly scattering identical nanoparticles. The ultra−short, ultra−bright X−ray pulses provide snapshots of the randomly oriented particles frozen in time, and terminate before the onset of structural damage. As signal strength diminishes for small particles, the synthesis of a three−dimensional diffraction volume requires simultaneous involvement of all data. Here we report the first application of a three−dimensional spatial frequency correlation analysis to carry out this synthesis from noisy single−particle femtosecond X−ray diffraction patterns of nearly identical samples in random and unknown orientations, collected at the Linac Coherent Light Source. Our demonstration uses unsupported test particles created via aerosol self−assembly, and composed of two polystyrene spheres of equal diameter. The correlation analysis avoids the need for orientation determination entirely. This method may be applied to the structural determination of biological macromolecules in solution

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Language(s): eng - English
 Dates: 2012-05-242012-11-142012-12-11
 Publication Status: Published in print
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 Rev. Type: Peer
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 3 Sequence Number: 1276 Start / End Page: 1 - 7 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723