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  Structural Organization of DNA in Chlorella Viruses

Wulfmeyer, T., Polzer, C., Hiepler, G., Hamacher, K., Shoeman, R. L., Dunigan, D. D., et al. (2012). Structural Organization of DNA in Chlorella Viruses. PLoS One, 7(2): e30133, pp. 1-10. doi:10.1371/journal.pone.0030133.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-1FB1-8 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-1FB2-6
Genre: Journal Article
Alternative Title : Structural Organization of DNA in Chlorella Viruses

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 Creators:
Wulfmeyer, Timo, Author
Polzer, Christian, Author
Hiepler, Gregor, Author
Hamacher, Kay, Author
Shoeman, Robert L.1, Author           
Dunigan, David D., Author
Van Etten, James L., Author
Lolicato, Marco, Author
Moroni, Anna, Author
Thiel, Gerhard, Author
Meckel, Tobias, Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Chlorella viruses have icosahedral capsids with an internal membrane enclosing their large dsDNA genomes and associated proteins. Their genomes are packaged in the particles with a predicted DNA density of ca. 0.2 bp nm−3. Occasionally infection of an algal cell by an individual particle fails and the viral DNA is dynamically ejected from the capsid. This shows that the release of the DNA generates a force, which can aid in the transfer of the genome into the host in a successful infection. Imaging of ejected viral DNA indicates that it is intimately associated with proteins in a periodic fashion. The bulk of the protein particles detected by atomic force microscopy have a size of ˜60 kDa and two proteins (A278L and A282L) of about this size are among 6 basic putative DNA binding proteins found in a proteomic analysis of DNA binding proteins packaged in the virion. A combination of fluorescence images of ejected DNA and a bioinformatics analysis of the DNA reveal periodic patterns in the viral DNA. The periodic distribution of GC rich regions in the genome provides potential binding sites for basic proteins. This DNA/protein aggregation could be responsible for the periodic concentration of fluorescently labeled DNA observed in ejected viral DNA. Collectively the data indicate that the large chlorella viruses have a DNA packaging strategy that differs from bacteriophages; it involves proteins and share similarities to that of chromatin structure in eukaryotes

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Language(s): eng - English
 Dates: 2011-09-272011-12-092012-02-16
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 664421
DOI: 10.1371/journal.pone.0030133
URI: http://tubiblio.ulb.tu-darmstadt.de/56679/
URI: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0030133
URI: http://www.plosone.org/article/fetchObjectAttachment.action;jsessionid=219D4CCECBAB66CC337382E27798BDF7?uri%3Dinfo%253Adoi%252F10.1371%252Fjournal.pone.0030133%26representation%3DPDF
Other: 7765
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 7 (2) Sequence Number: e30133 Start / End Page: 1 - 10 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850