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Free keywords:
crystal mounting; data collection
Abstract:
A new chip−based crystal−mounting approach for rapid room−temperature data
collection from numerous crystals is described. This work was motivated by the
recent development of X−ray free−electron lasers. These novel sources deliver
very intense femtosecond X−ray pulses that promise to yield high−resolution
diffraction data of nanocrystals before their destruction by radiation damage.
Thus, the concept of ‘diffraction before destruction' requires rapid replenishment
of the sample for each exposure. The chip promotes the self−assembly of an
array of protein crystals on a surface. Rough features on the surface cause the
crystals to adopt random orientations, allowing efficient sampling of reciprocal
space
