English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
 Local TagsRelease HistoryDetailsSummary
  Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse

Storck, T., Storck, U., Kolhekar, R., Sprengel, R., & Seeburg, P. H. (1996). Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse. Nucleic Acids Research (London), 24(22), 4594-4596. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/8948655.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-213B-A Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002A-FFD9-B
Genre: Journal Article
Alternative Title : Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse

Files

show Files
hide Files
:
NuclAcidRes_24_1996_4594.pdf (Any fulltext), 30KB
 
File Permalink:
-
Name:
NuclAcidRes_24_1996_4594.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://nar.oupjournals.org/cgi/reprint/24/22/4594 (Any fulltext)
Description:
-
OA-Status:
Locator:
http://dx.doi.org/10.1093/nar/24.22.4373 (Any fulltext)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Storck, Thorsten, Author
Storck, U., Author
Kolhekar, Rohini, Author
Sprengel, Rolf1, Author           
Seeburg, Peter H.1, Author           
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

Content

show
hide
Free keywords: -
 Abstract: Targeting vectors for embryonic stem (ES) cells typically contain a mouse gene segment of >7 kb with the neo gene inserted for positive selection of the targeting event. More complex targeting vectors carry additional genetic elements (e.g. lacZ, loxP, point mutations). Here we use homologous recombination in yeast to construct targeting vectors for the incorporation of genetic elements (GEs) into mouse genes. The precise insertion of GEs into any position of a mouse gene segment cloned in an Escherichia coli/yeast shuttle vector is directed by short recombinogenic arms (RAs) flanking the GEs. In this way, complex targeting vectors can be engineered with considerable ease and speed, obviating extensive gene mapping in search for suitable restriction sites

Details

show
hide
Language(s): eng - English
 Dates: 1996-10-071996-07-211996-10-071996-11-15
 Publication Status: Issued
 Pages: 3
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 666427
URI: http://www.ncbi.nlm.nih.gov/pubmed/8948655
URI: http://nar.oupjournals.org/cgi/content/full/24/22/4594
URI: http://nar.oupjournals.org/cgi/reprint/24/22/4594
Other: 4580
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nucleic Acids Research (London)
  Other : Nucleic Acids Res
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford : Oxford University Press
Pages: - Volume / Issue: 24 (22) Sequence Number: - Start / End Page: 4594 - 4596 Identifier: ISSN: 0305-1048
CoNE: https://pure.mpg.de/cone/journals/resource/110992357379342