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  Optogenetic dissection of neuronal circuits in zebrafish using viral gene transfer and the Tet system

Zhu, P., Narita, Y., Bundschuh, S., Fajardo, O., Schärer, Y.-P.-Z., Chattopadhyaya, B., et al. (2009). Optogenetic dissection of neuronal circuits in zebrafish using viral gene transfer and the Tet system. Frontiers in neural circuits, 3: 21, pp. 1-12. doi:10.3389/neuro.04.021.2009.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-232B-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-500E-9
Genre: Journal Article
Alternative Title : Optogenetic dissection of neuronal circuits in zebrafish using viral gene transfer and the Tet system

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 Creators:
Zhu, Peixin1, Author              
Narita, Yuichi, Author
Bundschuh, Sebastian2, Author              
Fajardo, Otto, Author
Schärer, Yan-Ping Zhang, Author
Chattopadhyaya, Bidisha2, Author              
Bouldoires, Estelle Arn, Author
Stepien, Anna Ewa, Author
Deisseroth, Karl, Author
Arber, Silvia, Author
Sprengel, Rolf1, Author              
Rijli, Filippo M., Author
Friedrich, Rainer W.2, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: zebrafish, Tet system, viral gene transfer, channelrhodopsin, olfactory bulb, optogenetics, multiphoton
 Abstract: The conditional expression of transgenes at high levels in sparse and specific populations of neurons is important for high-resolution optogenetic analyses of neuronal circuits. We explored two complementary methods, viral gene delivery and the iTet-Off system, to express transgenes in the brain of zebrafish. High-level gene expression in neurons was achieved by Sindbis and Rabies viruses. The Tet system produced strong and specific gene expression that could be modulated conveniently by doxycycline. Moreover, transgenic lines showed expression in distinct, sparse and stable populations of neurons that appeared to be subsets of the neurons targeted by the promoter driving the Tet-activator. The Tet system therefore provides the opportunity to generate libraries of diverse expression patterns similar to gene trap approaches or the thy-1 promoter in mice, but with the additional possibility to pre-select cell types of interest. In transgenic lines expressing channelrhodopsin-2, action potential firing could be precisely controlled by two-photon stimulation at low laser power, presumably because the expression levels of the Tet-controlled genes were high even in adults. In channelrhodopsin-2-expressing larvae, optical stimulation with a single blue LED evoked distinct swimming behaviors including backward swimming. These approaches provide new opportunities for the optogenetic dissection of neuronal circuit structure and function.

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Language(s): eng - English
 Dates: 2009-09-302009-11-272009-12-112009-12-11
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 664657
DOI: 10.3389/neuro.04.021.2009
URI: http://www.ncbi.nlm.nih.gov/pubmed/20126518
Other: 7553
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Title: Frontiers in neural circuits
  Alternative Title : Frontiers in neural circuits
Source Genre: Journal
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Pages: - Volume / Issue: 3 Sequence Number: 21 Start / End Page: 1 - 12 Identifier: ISSN: 1662-5110