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  Towards automatic protein backbone assignment using proton-detected 4D solid-state NMR data.

Xiang, S. Q., Chevelkov, V., Becker, S., & Lange, A. (2014). Towards automatic protein backbone assignment using proton-detected 4D solid-state NMR data. Journal of Biomolecular NMR, 60(2-3), 85-90. doi:10.1007/s10858-014-9859-6.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-246E-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-DD39-C
Genre: Journal Article

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 Creators:
Xiang, S. Q., Author
Chevelkov, V.1, Author              
Becker, S.1, Author              
Lange, A.1, Author              
Affiliations:
1Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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 Abstract: We introduce an efficient approach for sequential protein backbone assignment based on two complementary proton-detected 4D solid-state NMR experiments that correlate HNi /Ni with CAi/COi or CAi−1/COi−1. The resulting 4D spectra exhibit excellent sensitivity and resolution and are amenable to (semi-)automatic assignment approaches. This strategy allows to obtain sequential connections with high confidence as problems related to peak overlap and multiple assignment possibilities are avoided. Non-uniform sampling schemes were implemented to allow for the acquisition of 4D spectra within a few days. Rather moderate hardware requirements enable the successful demonstration of the method on deuterated type III secretion needles using a 600 MHz spectrometer at a spinning rate of 25 kHz.

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Language(s): eng - English
 Dates: 2014-09-062014-11
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s10858-014-9859-6
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Title: Journal of Biomolecular NMR
Source Genre: Journal
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Pages: - Volume / Issue: 60 (2-3) Sequence Number: - Start / End Page: 85 - 90 Identifier: -