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  Assembly, characterization, and delivery of quantum dot labeled biotinylated lipid particles.

Sigot, V. (2014). Assembly, characterization, and delivery of quantum dot labeled biotinylated lipid particles. In A. Fontes, & B. S. Santos (Eds.), Quantum dots: Applications in biology (pp. 113-127). New York, N.Y.: Springer. doi:10.1007/978-1-4939-1280-3_9.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-34F6-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-1A7C-9
Genre: Book Chapter

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2070913.pdf (Publisher version), 722KB
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 Creators:
Sigot, V.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: Biotinylated lipid particles; Quantum dots; Epidermal growth factor; Targeted delivery; Detergent dialysis; Confocal fluorescence microscopy
 Abstract: Lipid nanoparticles composed of mixtures of PEGylated-lipids; cationic and neutral lipids prepared by detergent dialysis can encapsulate biological active molecules and show considerable potential as systemic therapeutic agents. Addition of biotinylated lipids to this formulation allows surface modification of these particles with a suitable ligand or probe conjugated to streptavidin for specific cell targeting. Monitoring long circulating particles and cellular uptake requires stable and bright fluorescent probes. Quantum dots (QDs) constitute a relatively new class of fluorescent probes that overcome the limitations of organic fluorophores in biological imaging applications. Here, a protocol for the encapsulation of QD655 (red) in biotinylated lipid particles (BLPs) prepared by a detergent dialysis technique is presented followed by characterization of the loaded liposomal vehicles. Then, a protocol for BLPs surface modification via biotin-streptavidin linkage with preformed complexes of ligand-QD525 (green) for specific cell targeting of the nanoparticle is detailed. Conditions for cell binding and uptake of two colors QD labeled BLPs as well as basic microscopic settings for confocal live cell imaging are described.

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Language(s): eng - English
 Dates: 2014-07-182014
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/978-1-4939-1280-3_9
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Title: Quantum dots: Applications in biology
Source Genre: Book
 Creator(s):
Fontes, A., Editor
Santos, B. S., Editor
Affiliations:
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Publ. Info: New York, N.Y. : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 113 - 127 Identifier: ISBN: 978-1-4939-1279-7

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Title: Methods in Molecular Biology
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Pages: - Volume / Issue: 1199 Sequence Number: - Start / End Page: - Identifier: -