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  Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells

Klingner, C., Cherian, A. V., Fels, J., Diesinger, P. M., Aufschnaiter, R., Maghelli, N., et al. (2014). Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells. JOURNAL OF CELL BIOLOGY, 207(1), 107-121. doi:10.1083/jcb.201402037.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-39C3-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-39C4-6
Genre: Journal Article

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 Creators:
Klingner, Christoph1, Author              
Cherian, Anoop V.1, Author              
Fels, Johannes2, Author
Diesinger, Philipp M.2, Author
Aufschnaiter, Roland1, Author              
Maghelli, Nicola2, Author
Keil, Thomas3, Author              
Beck, Gisela1, Author              
Tolic-Norrelykke, Iva M.2, Author
Bathe, Mark2, Author
Wedlich-Soldner, Roland1, Author              
Affiliations:
1Wedlich-Söldner, Roland / Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565174              
2external, ou_persistent22              
3Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: EPIDERMAL-GROWTH-FACTOR; SINGLE-MOLECULE TECHNIQUES; SCAFFOLDING PROTEIN EBP50; INTESTINAL BRUSH-BORDER; MESENCHYMAL TRANSITION; PLASMA-MEMBRANE; LEADING-EDGE; ERM PROTEINS; STEM-CELLS; F-ACTIN
 Abstract: Although cortical actin plays an important role in cellular mechanics and morphogenesis, there is surprisingly little information on cortex organization at the apical surface of cells. In this paper, we characterize organization and dynamics of microvilli (MV) and a previously unappreciated actomyosin network at the apical surface of Madin-Darby canine kidney cells. In contrast to short and static MV in confluent cells, the apical surfaces of nonconfluent epithelial cells (ECs) form highly dynamic protrusions, which are often oriented along the plane of the membrane. These dynamic MV exhibit complex and spatially correlated reorganization, which is dependent on myosin II activity. Surprisingly, myosin II is organized into an extensive network of filaments spanning the entire apical membrane in nonconfluent ECs. Dynamic MV, myosin filaments, and their associated actin filaments form an interconnected, prestressed network. Interestingly, this network regulates lateral mobility of apical membrane probes such as integrins or epidermal growth factor receptors, suggesting that coordinated actomyosin dynamics contributes to apical cell membrane organization.

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Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: ISI: 000343194900011
DOI: 10.1083/jcb.201402037
 Degree: -

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Title: JOURNAL OF CELL BIOLOGY
Source Genre: Journal
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Publ. Info: 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA : ROCKEFELLER UNIV PRESS
Pages: - Volume / Issue: 207 (1) Sequence Number: - Start / End Page: 107 - 121 Identifier: ISSN: 0021-9525