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  NMR-based detection of hydrogen/deuterium exchange in liposome-embedded membrane proteins.

Yao, X., Dürr, U. H. N., Gattin, Z., Laukat, Y., Narayanan, R., Brückner, A. K., et al. (2014). NMR-based detection of hydrogen/deuterium exchange in liposome-embedded membrane proteins. PLOS One, 9(11): e112374. doi:10.1371/journal.pone.0112374.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-4000-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CCFD-1
Genre: Journal Article

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 Creators:
Yao, X.1, Author              
Dürr, U. H. N.1, Author              
Gattin, Z.2, Author              
Laukat, Y.3, Author              
Narayanan, R.L.1, Author              
Brückner, A. K., Author
Meisinger, C., Author
Lange, A., Author
Becker, S.3, Author              
Zweckstetter, M.1, Author              
Affiliations:
1Research Group of Protein Structure Determination using NMR, MPI for Biophysical Chemistry, Max Planck Society, ou_578571              
2Research Group of Solid-state NMR, MPI for biophysical chemistry, Max Planck Society, ou_persistent35              
3Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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 Abstract: Membrane proteins play key roles in biology. Determination of their structure in a membrane environment, however, is highly challenging. To address this challenge, we developed an approach that couples hydrogen/deuterium exchange of membrane proteins to rapid unfolding and detection by solution-state NMR spectroscopy. We show that the method allows analysis of the solvent protection of single residues in liposome-embedded proteins such as the 349-residue Tom40, the major protein translocation pore in the outer mitochondrial membrane, which has resisted structural analysis for many years.

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Language(s): eng - English
 Dates: 2014-11-06
 Publication Status: Published online
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0112374
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Title: PLOS One
Source Genre: Journal
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Pages: 8 Volume / Issue: 9 (11) Sequence Number: e112374 Start / End Page: - Identifier: -