English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  EPR moments for site-directed spin-labelling.

Marsh, D. (2014). EPR moments for site-directed spin-labelling. Journal of Magnetic Resonance, 248, 66-70. doi:10.1016/j.jmr.2014.09.006.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-40AA-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-606E-0
Genre: Journal Article

Files

show Files
hide Files
:
2073071.pdf (Publisher version), 410KB
 
File Permalink:
-
Name:
2073071.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Marsh, D.1, Author              
Affiliations:
1Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

Content

show
hide
Free keywords: EPR moments; Spin label; SDSL; Rotational diffusion; Order parameter
 Abstract: The absolute-value first moment and second moment of nitroxide EPR absorption spectra, which can be used for systematic analysis in site-directed spin-labelling, are analysed in terms of the rotational mobility and angular motional amplitude of the spin probe. Spectral simulations with the stochastic Liouville equation are used to give calibrations of second and absolute-value first moments as a function of isotropic rotational correlation time tauR and axial order parameter Szz, over the entire range of sensitivity to these parameters. Both moments are additive for multicomponent spectra, e.g., for multiple conformations. The absolute-value first moment has the advantage over the central line width in that it does not overemphasize fast motional components, and over the second moment in that it is less sensitive to noise in the outer wings of the spectrum and to baseline cutoff.

Details

show
hide
Language(s): eng - English
 Dates: 2014-09-222014-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.jmr.2014.09.006
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Magnetic Resonance
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 248 Sequence Number: - Start / End Page: 66 - 70 Identifier: -