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  Dimensions and ion selectivity of recombinant AMPA and kainate receptor channels and their dependence on Q/R site residues

Burnashev, N., Villarroel, A., & Sakmann, B. (1996). Dimensions and ion selectivity of recombinant AMPA and kainate receptor channels and their dependence on Q/R site residues. The Journal of Physiology - London, 496, 165-173.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-4167-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-4168-2
Genre: Journal Article
Alternative Title : Dimensions and ion selectivity of recombinant AMPA and kainate receptor channels and their dependence on Q/R site residues

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JPhysiol_496_1996_165.pdf (Any fulltext), 2MB
 
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Burnashev, Nail1, Author              
Villarroel, Alfredo1, Author              
Sakmann, Bert1, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: 1. Recombinant alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor (AMPAR) subunits (GluR-A or GluR-B) and kainate receptor (KAR) subunit (GluR-6) in their unedited (Q)- and edited (R)-forms were expressed in HEK 293 cells. To estimate the dimensions of the narrow portion of these channels, biionic reversal potentials for organic cations of different mean diameters were determined with Cs+ as the internal reference ion. 2. Homomeric channels assembled from Q-form subunits were cation selective. The relation between the relative permeability and the mean size of different organic cations suggests that the diameter of the narrow portion of Q-form channels is approximately 0.78 nm for AMPAR and 0.75 nm for KAR channels. 3. Homomeric channels assembled from R-form subunits were permeant for anions and cations. When probed with CsC1 gradients the relative chloride permeability (PC1/PCs) was estimated as 0.14 for GluR-B(R) and 0.74 for GluR-6(R)-subunit channels. The permeability versus mean size relation for large cations measured with the weakly permeant F- as anion, indicates that for the R-form KAR channels the apparent pore diameter is close to 0.76 nm. 4. Heteromeric AMPAR and KAR channels co-assembled from Q- and R-form subunits were cation selective. The diameter of the narrow portion of these channels is estimated to be in the range between 0.70 and 0.74 nm. 5. The results indicated that the diameters of the narrow portion of AMPAR and KAR channels of different subunit composition and of widely different ion selectivity are comparable. Therefore, the differences in the anion versus cation selectivity, in Ca2+ permeability and in channel conductance are likely to be determined by the difference in charge density of the channel.

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Language(s): eng - English
 Dates: 1996-06-191996-07-301996
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 666457
Other: 4550
 Degree: -

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Title: The Journal of Physiology - London
Source Genre: Journal
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Publ. Info: London : Cambridge University Press
Pages: - Volume / Issue: 496 Sequence Number: - Start / End Page: 165 - 173 Identifier: ISSN: 0022-3751
CoNE: https://pure.mpg.de/cone/journals/resource/954925334693_2