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  Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis

Schulze, P., Schüttpelz, M., Sauer, M., & Belder, D. (2007). Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis. Lab on a Chip, 7(12), 1841-1844. doi:10.1039/B710762E.

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 Creators:
Schulze, Philipp1, Author           
Schüttpelz, Mark2, Author
Sauer, Markus2, Author
Belder, Detlev1, Author           
Affiliations:
1Institute of Analytical Chemistry, University of Leipzig, Linnéstr. 3, Leipzig, Germany, ou_persistent22              
2Applied Laser Physics and Laser Spectroscopy, University of Bielefeld, Universitätsstraße 25, Bielefeld, Germany, ou_persistent22              

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 Abstract: Two photon excited (TPE) fluorescence detection was applied to native fluorescence detection of aromatics in microchip electrophoresis (MCE). This technique was evaluated as an alternative to common one photon excitation in the deep UV spectral range. TPE enables fluorescence detection of unlabeled aromatic compounds, even in non-deep UV-transparent microfluidic chips. In this study, we demonstrate the proof of concept of native TPE fluorescence detection of small aromatics in commercial microfluidic glass chips. Label-free TPE fluorescence detection of native proteins and small aromatics in MCE was achieved within the micromolar concentration range, utilising 420 nm excitation light.

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Language(s): eng - English
 Dates: 2007-07-172007-09-192007-10-102007-12-01
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1039/B710762E
 Degree: -

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Title: Lab on a Chip
  Other : Lab Chip
Source Genre: Journal
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Publ. Info: Cambridge : Royal Society of Chemistry
Pages: - Volume / Issue: 7 (12) Sequence Number: - Start / End Page: 1841 - 1844 Identifier: ISSN: 1473-0197
CoNE: https://pure.mpg.de/cone/journals/resource/14730197