English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Structural determinants of channel conductance in fetal and adult rat muscle acetylcholine receptors

Herlitze, S., Villarroel, A., Witzemann, V., Koenen, M., & Sakmann, B. (1996). Structural determinants of channel conductance in fetal and adult rat muscle acetylcholine receptors. Journal of Physiology-London, 492(3), 775-788. Retrieved from http://www.jphysiol.org/cgi/content/abstract/492/3/775?maxtoshow%3D%26HITS%3D10%26hits%3D10%26RESULTFORMAT%3D%26searchid%3D1021620028575_85%26stored_search%3D%26FIRSTINDEX%3D0%26volume%3D492%26firstpage%3D775%26fdate%3D1%2F1%2F1996%26tdate%3D12%2F31%2F1996%26journalcode%3Djphysiol%3A.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-42FD-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-42FE-C
Genre: Journal Article
Alternative Title : Structural determinants of channel conductance in fetal and adult rat muscle acetylcholine receptors

Files

show Files
hide Files
:
JPhysiol_492_1996_775.pdf (Any fulltext), 3MB
 
File Permalink:
-
Name:
JPhysiol_492_1996_775.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Herlitze, Stefan1, Author              
Villarroel, Alfredo1, Author              
Witzemann, Veit1, 2, Author              
Koenen, Michael1, 2, Author              
Sakmann, Bert1, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

Content

show
hide
Free keywords: -
 Abstract: 1. The structural basis of the developmentally regulated increase in endplate channel conductance in rat, where the gamma−subunit of the fetal muscle acetylcholine receptor (gamma−AChR) is replaced by the epsilon−subunit in the adult muscle receptor (epsilon−AChR), was investigated by analysing the structure of gamma− and epsilon−subunit genes and by expressing recombinant AChR channels of different molecular composition in Xenopus oocytes and measuring their single− channel conductance. 2. The gamma− and epsilon−subunit genes each have twelve exons. In both subunits, the four homologous segments, designated M1, M2, M3 and M4, which are thought to contribute to the formation of the pore, are encoded by four separate exons, E7, E8, E9 and E12. 3. Chimaeric epsilon(gamma)− or gamma(epsilon)−subunits were constructed from the parental epsilon− and gamma−subunits, respectively. Exchanging the four hydrophobic segments (M1−M4) of the gamma−subunit for those of the epsilon−subunit and vice versa completely reversed the difference in conductance between gamma−AChR and epsilon−AChR channels. 4. Effects of single− and multiple−point mutations in M1−M4 segments of gamma− and epsilon−subunits indicate that the major determinants of the difference in conductance between fetal and adult endplate channels are located in the M2 segment. The key differences are the exchange of alanine/threonine (γ−subunit) for serine/isoleucine (ε−subunit) in M2, and the lysine (gamma− subunit) for glutamine (epsilon−subunit) exchanges in the regions flanking the M2 segment

Details

show
hide
Language(s): eng - English
 Dates: 1995-08-091995-11-301996
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Physiology-London
  Other : J. Physiol.-London
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Cambridge University Press
Pages: - Volume / Issue: 492 (3) Sequence Number: - Start / End Page: 775 - 788 Identifier: ISSN: 0022-3751
CoNE: https://pure.mpg.de/cone/journals/resource/954925334693_1