Generation of targeted mouse mutants by embryo microinjection of TALENs

Wefers, Benedikt; Ortiz, Oskar; Wurst, Wolfgang; Kuehn, Ralf

doi:10.1016/j.ymeth.2014.01.002

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Generation of targeted mouse mutants by embryo microinjection of TALENs

Wefers, B., Ortiz, O., Wurst, W., & Kuehn, R. (2014). Generation of targeted mouse mutants by embryo microinjection of TALENs. METHODS, 69(1), 94-101. doi:10.1016/j.ymeth.2014.01.002.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-6104-6 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-6105-4

Genre: Journal Article

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Creators:
Wefers, Benedikt¹, Author
Ortiz, Oskar¹, Author
Wurst, Wolfgang^{2, 3}, Author
Kuehn, Ralf¹, Author

Affiliations:
1external, ou_persistent22
2Max Planck Institute of Psychiatry, Max Planck Society, ou_1607137
3Institute of Develomental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, ou_persistent22

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Abstract: Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes. (C) 2014 Elsevier Inc. All rights reserved.

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Language(s): eng - English

Dates: Date issued: 2014

Publication Status: Issued

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Identifiers: ISI: 000341803500013
DOI: 10.1016/j.ymeth.2014.01.002

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Title: METHODS

Source Genre: Journal

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Publ. Info: Amsterdam : Elsevier

Pages: - Volume / Issue: 69 (1) Sequence Number: - Start / End Page: 94 - 101 Identifier: ISSN: 1046-2023