English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Fractionation profiling: a fast and versatile approach for mapping vesicle proteomes and protein-protein interactions

Borner, G. H. H., Hein, M. Y., Hirst, J., Edgar, J. R., Mann, M., & Robinson, M. S. (2014). Fractionation profiling: a fast and versatile approach for mapping vesicle proteomes and protein-protein interactions. MOLECULAR BIOLOGY OF THE CELL, 25(20), 3178-3194. doi:10.1091/mbc.E14-07-1198.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Borner, Georg H. H.1, Author              
Hein, Marco Y.1, Author              
Hirst, Jennifer2, Author
Edgar, James R.2, Author
Mann, Matthias1, Author              
Robinson, Margaret S.2, Author
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

Content

show
hide
Free keywords: CLATHRIN-COATED VESICLES; E3 UBIQUITIN LIGASE; INDEPENDENT ENDOCYTOSIS; ACCESSORY PROTEINS; COMPLEX; QUANTIFICATION; LOCALIZATION; COMPONENTS; DROSOPHILA; TRANSPORT
 Abstract: We developed "fractionation profiling," a method for rapid proteomic analysis of membrane vesicles and protein particles. The approach combines quantitative proteomics with subcellular fractionation to generate signature protein abundance distribution profiles. Functionally associated groups of proteins are revealed through cluster analysis. To validate the method, we first profiled >3500 proteins from HeLa cells and identified known clathrin-coated vesicle proteins with >90% accuracy. We then profiled >2400 proteins from Drosophila S2 cells, and we report the first comprehensive insect clathrin-coated vesicle proteome. Of importance, the cluster analysis extends to all profiled proteins and thus identifies a diverse range of known and novel cytosolic and membrane-associated protein complexes. We show that it also allows the detailed compositional characterization of complexes, including the delineation of subcomplexes and subunit stoichiometry. Our predictions are presented in an interactive database. Fractionation profiling is a universal method for defining the clathrin-coated vesicle proteome and may be adapted for the analysis of other types of vesicles and particles. In addition, it provides a versatile tool for the rapid generation of large-scale protein interaction maps.

Details

show
hide
Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000343881000007
DOI: 10.1091/mbc.E14-07-1198
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: MOLECULAR BIOLOGY OF THE CELL
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA : AMER SOC CELL BIOLOGY
Pages: - Volume / Issue: 25 (20) Sequence Number: - Start / End Page: 3178 - 3194 Identifier: ISSN: 1059-1524