English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Fifteen Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

Mann, M. (2014). Fifteen Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC). In Methods in Molecular Biology; Vol. 1188 (pp. 1-7). 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA: HUMANA PRESS INC.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Mann, Matthias1, Author              
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

Content

show
hide
Free keywords: QUANTITATIVE PROTEOMICS; MASS-SPECTROMETRY; IN-VIVO; PEPTIDE IDENTIFICATION; PROTEIN INTERACTIONS; SIGNALING NETWORKS; QUANTIFICATION; CONVERSION; ARGININE; ACCURATEStable isotope labeling; Proteomics; Quantitation; Posttranslational modifications; Mass spectrometry; Electrospray; Protein analysis;
 Abstract: Here I describe the history of the Stable Isotope Labeling by Amino Acids in Cell culture (SILAC) technology. Although published in 2002, it had already been developed and used in my laboratory for a number of years. From the beginning, it was applied to challenging problems in cell signaling that were considered out of reach for proteomics at the time. It was also used to pioneer proteomic interactomics, time series and dynamic posttranslational modification studies. While initially developed for metabolically accessible systems, such as cell lines, it was subsequently extended to whole animal labeling as well as to clinical applications-in the form or spike-in or super-SILAC. New formats and applications for SILAC labeling continue to be developed, for instance for protein-turnover studies.

Details

show
hide
Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Methods in Molecular Biology; Vol. 1188
  Alternative Title : STABLE ISOTOPE LABELING BY AMINO ACIDS IN CELL CULTURE (SILAC): METHODS AND PROTOCOLS
  Alternative Title :
Source Genre: Series
 Creator(s):
Affiliations:
Publ. Info: 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA : HUMANA PRESS INC
Pages: - Volume / Issue: 1188 Sequence Number: - Start / End Page: 1 - 7 Identifier: ISSN: 1064-3745
ISBN: 978-1-4939-1142-4; 978-1-4939-1141-7