English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems

Zieske, K., & Schwille, P. (2014). Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems. eLife, 3: e03949. doi:10.7554/eLife.03949.

Item is

Files

show Files
hide Files
:
elife-03949-v2.pdf (Publisher version), 4MB
Name:
elife-03949-v2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Zieske, Katja1, Author              
Schwille, Petra1, Author              
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

Content

show
hide
Free keywords: DIVISION-SITE SELECTION; SHAPED ESCHERICHIA-COLI; TO-POLE OSCILLATION; BACILLUS-SUBTILIS; CYTOPLASMIC VOLUME; INHIBITOR MINC; FISSION YEAST; IN-VITRO; FTSZ; MEMBRANE
 Abstract: Intracellular protein gradients are significant determinants of spatial organization. However, little is known about how protein patterns are established, and how their positional information directs downstream processes. We have accomplished the reconstitution of a protein concentration gradient that directs the assembly of the cell division machinery in E.coli from the bottom-up. Reconstituting self-organized oscillations of MinCDE proteins in membrane-clad soft-polymer compartments, we demonstrate that distinct time-averaged protein concentration gradients are established. Our minimal system allows to study complex organizational principles, such as spatial control of division site placement by intracellular protein gradients, under simplified conditions. In particular, we demonstrate that FtsZ, which marks the cell division site in many bacteria, can be targeted to the middle of a cell-like compartment. Moreover, we show that compartment geometry plays a major role in Min gradient establishment, and provide evidence for a geometry-mediated mechanism to partition Min proteins during bacterial development.

Details

show
hide
Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
 Pages: 47
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000343409900002
DOI: 10.7554/eLife.03949
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: eLife
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 3 Sequence Number: e03949 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X