English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A new weakly basic amino-reactive fluorescent label for use in isoelectric focusing and chip electrophoresis

Schulze, P., Link, M., Schulze, M., Thuermann, S., Wolfbeis, O. S., & Belder, D. (2010). A new weakly basic amino-reactive fluorescent label for use in isoelectric focusing and chip electrophoresis. Electrophoresis, 31(16), 2749-2753. doi:10.1002/elps.201000007.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Schulze, Philipp1, Author           
Link, Martin2, Author
Schulze, Marcel1, Author
Thuermann, Sebastian1, Author
Wolfbeis, Otto S.2, Author
Belder, Detlev1, Author           
Affiliations:
1Institute of Analytical Chemistry, University of Leipzig, 04229, Leipzig, Germany, ou_persistent22              
2Institute of Analytical Chemistry, Chemo‐ and Biosensors, University of Regensburg, Regensburg, Germany, ou_persistent22              

Content

show
hide
Free keywords: CIEF; Fluorescence marker; MCE; Luminescence detection; Protein labeling
 Abstract: In this study, we present a novel amino-reactive fluorescence marker (referred to as UR-431), which is well suited for electrophoretic techniques. A main feature of this marker is its weakly basic behavior when conjugated to analytes. Labeled primary amines exhibit a positive net charge and accordingly a cathodic mobility below a pH of 2.4. The label features a pH-independent fluorescence emission and is thus very interesting for electrophoretic applications such as IEF. The absorption maximum of this yellow daylight chromophore is at 431 nm, whereas fluorescence emission peaks at 537 nm (quantum yield≈0.1). The label was successfully conjugated to amines, peptides and proteins and separated via CE and MCE. The on-chip detection limit of labeled lysine using a mercury-lamp-based fluorescence microscope was determined as 12 nM. An important feature of the new label is that it effects only a subtle change of the pI of proteins compared with common anionic labels, e.g. FITC. pI values of proteins were investigated by comparing native proteins and labeled proteins in CIEF. UR-431 was also applied to sensitive detection of amines and peptides in MCE.

Details

show
hide
Language(s): eng - English
 Dates: 2010-01-062020-05-052010-07-192010-08-01
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/elps.201000007
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Electrophoresis
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim, Germany : Verlag Chemie,
Pages: - Volume / Issue: 31 (16) Sequence Number: - Start / End Page: 2749 - 2753 Identifier: ISSN: 0173-0835
CoNE: https://pure.mpg.de/cone/journals/resource/954925486765