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  Reduced intracellular mobility underlies manganese relaxivity in mouse brain in vivo: MRI at 2.35 and 9.4 T.

Watanabe, T., Frahm, J., & Michaelis, T. (2015). Reduced intracellular mobility underlies manganese relaxivity in mouse brain in vivo: MRI at 2.35 and 9.4 T. Brain Structure and Function, 220(3), 1529-1538. doi:10.1007/s00429-014-0742-8.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-4654-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2249-D
Genre: Journal Article

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 Creators:
Watanabe, T.1, Author              
Frahm, J.1, Author              
Michaelis, T.1, Author              
Affiliations:
1Biomedical NMR Research GmbH, MPI for biophysical chemistry, Max Planck Society, ou_578634              

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Free keywords: Brain mapping, Contrast media, Gadolinium-DTPA, Intraventricular injections, Manganese chloride, Subcutaneous injections
 Abstract: Using T1-weighted MRI at two different magnetic field strengths, the enhanced longitudinal relaxivity due to paramagnetic manganese ions in mouse brain in vivo is shown to reflect reduced intracellular mobility. One day after systemic administration of manganese chloride, increases of the longitudinal relaxation rate DR1 in several brain regions are significantly higher at 2.35 T than at 9.4 T. The corresponding relaxivity ratios 100r1/400r1 = 100DR1/400DR1 range from 2.4 (striatum) to 4.4 (cerebellar cortex). In contrast, the DR1 values after intraventricular administration of gadolinium-DTPA (Gd- DTPA) are not significantly different between both field strengths yielding 100r1/400r1 ratios from 1.0 to 1.1. The same observation holds true for manganese and Gd-DTPA relaxivities in aqueous solution. The pronounced field strength dependence of manganese relaxivities indicates a reduced mobility of manganese ions in vivo by confinement to a viscous fluid compartment and/or due to macromolecular binding. Moreover, preferential enhancement of nerve cell assemblies by manganese ions and the observation of additional contrast enhancement by magnetization transfer suggest an intracellular localization of manganese. This is further supported by a slow release of manganese from nerve cells postmortem, which occurs despite a high permeability of damaged cellular membranes as demonstrated by a rapid uptake of extracellular Gd-DTPA.

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Language(s): eng - English
 Dates: 2014-03-212015-05
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s00429-014-0742-8
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Title: Brain Structure and Function
Source Genre: Journal
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Pages: - Volume / Issue: 220 (3) Sequence Number: - Start / End Page: 1529 - 1538 Identifier: -