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  Expression of AMPA-glutamate receptor B subunit in rat hippocampal GABAergic neurons

Racca, C., Catania, M. V., Monyer, H., & Sakmann, B. (1996). Expression of AMPA-glutamate receptor B subunit in rat hippocampal GABAergic neurons. European Journal of Neuroscience: European Neuroscience Association, 8, 1580-1590. doi:10.1111/j.1460-9568.1996.tb01303.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-4922-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-4923-2
Genre: Journal Article
Alternative Title : Expression of AMPA-glutamate receptor B subunit in rat hippocampal GABAergic neurons

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EurJNeurosci_8_1996_1580.pdf (Any fulltext), 7MB
 
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 Creators:
Racca, Claudia, Author
Catania, Maria V., Author
Monyer, Hannah1, Author              
Sakmann, Bert2, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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Free keywords: glutamic acid decarboxylase, interneurons, digoxigenin, cRNA probes, hippocampus
 Abstract: The physiological properties of a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-type glutamate receptor (GIuR) channels are determined by their subunit composition. In particular, the expression of the GIuRB subunit determines the divalent ion selectivity of the channel. We studied the distribution of GIuR-B mRNA and protein in adult rat hippocampal GABAergic neurons combining non-radioactive in sifu hybridization with immunocytochemistry. The majority of GABAergic hippocampal neurons were GIuR-B mRNA-positive, but overall the levels of GIuR-B transcript were lower than in pyramidal and granule cells, which showed the highest hybridization signal. The different GIuR-B mRNA expression levels in GABAergic versus principal neurons were also observed at the protein level. There was a paucity of GIuR-B/C immunoreactivity in the vast majority of somata of the GABAergic neurons studied, which contrasted with the strong expression of GIuR-B/C proteins in the hippocampal principal neurons. Our results demonstrate the general low expression of the GIuR-B subunit mRNA and GIuR-B/C proteins in GABAergic hippocampal neurons. Considering the dominant role of the GIuR-B subunit in determining the divalent ion permeability of the receptor, it is likely

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Language(s): eng - English
 Dates: 1996-02-011995-10-261996-02-162006-04-071996
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 666432
Other: 4575
DOI: 10.1111/j.1460-9568.1996.tb01303.x
 Degree: -

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Title: European Journal of Neuroscience : European Neuroscience Association
  Other : Eur. J. Neurosci
Source Genre: Journal
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Publ. Info: Oxford, UK : Published on behalf of the European Neuroscience Association by Oxford University Press
Pages: - Volume / Issue: 8 Sequence Number: - Start / End Page: 1580 - 1590 Identifier: ISSN: 0953-816X
CoNE: https://pure.mpg.de/cone/journals/resource/954925575988