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  Mini-Tn7 vectors for studying post-transcriptional gene expression in Pseudomonas

Liu, Y., Rainey, P. B., & Zhang, X.-X. (2014). Mini-Tn7 vectors for studying post-transcriptional gene expression in Pseudomonas. Journal of Microbiological Methods, 107, 182-185. doi:10.1016/j.mimet.2014.10.015.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-498F-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-C2EB-D
Genre: Journal Article

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 Creators:
Liu, Yunhao, Author
Rainey, Paul B.1, Author              
Zhang, Xue-Xian, Author
Affiliations:
1External Scientific Member Group Experimental and Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445637              

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Free keywords: gene regulation; lacZ; noncoding RNAs; translational fusion; mini-Tn7; Pseudomonas
 Abstract: We describe the construction and validation of five mini-Tn7 vectors for analysis of post-transcriptional gene expression in Pseudomonas. Four vectors allowconstruction of translational fusions to β-galactosidase (lacZ),while the fifth is designed for functional analysis of noncoding RNA genes. Translational fusions can be constructed without a functional promoter in the vector or from an inducible promoter of either Ptac or PdctA. We show that promoterless fusions have value for determining levels of translation, whereas fusions to inducible promoters have utility in the analysis of mRNA-binding factors

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Language(s): eng - English
 Dates: 2014-10-262014-10-072014-10-262014-11-042014-12
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1016/j.mimet.2014.10.015
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Title: Journal of Microbiological Methods
Source Genre: Journal
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Publ. Info: Amsterdam, Netherlands : Elsevier
Pages: - Volume / Issue: 107 Sequence Number: - Start / End Page: 182 - 185 Identifier: ISSN: 0167-7012 (print)
ISSN: 0167-7012 (online)
ISSN: 1872-8359 (online)
CoNE: /journals/resource/954925483670