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  Enhanced signal intensity in matrix-free laser desorption ionization mass spectrometry by chemical modification of bionanostructures from diatom cell walls

Jaschinski, T., Thume, K., Klein, M., Richter, P., Popp, J., Svatoš, A., et al. (2014). Enhanced signal intensity in matrix-free laser desorption ionization mass spectrometry by chemical modification of bionanostructures from diatom cell walls. Rapid Communications in Mass Spectrometry, 28(13), 1521-1529. doi:10.1002/rcm.6930.

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 Creators:
Jaschinski, T., Author
Thume, K., Author
Klein, M., Author
Richter, P., Author
Popp, J., Author
Svatoš, Aleš1, Author           
Pohnert, Georg2, Author           
Affiliations:
1Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society, ou_421899              
2External Organizations, ou_persistent22              

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 Abstract: RATIONALE
Laser desorption ionization for mass spectrometric measurements (LDI MS) is supported by nanostructured materials. This technique helps to overcome known limitations of matrix‐assisted laser desorption/ionization (MALDI) and especially avoids interfering signals caused by matrix components. LDI can be supported by bionanostructures from the cell walls of diatoms. We explore how ionization efficiency can be improved by chemical modification of the cell walls.
METHODS

We introduce procedures to chemically modify these nanopatterned silicate structures using perfluorooctyldimethylchlorosilane or pentafluorophenylpropyldimethylchlorosilane. Using a conventional MALDI‐MS instrument we compare ionization using the novel materials with that of unmodified cell walls. The functionalized bionanomaterial is comprehensively evaluated for the use in LDI MS using a broad range of analytes and two commercial drugs.
RESULTS

Chemical modifications lead to materials that support LDI significantly better than unmodified diatom cell walls. LDI signal intensity was up to 25‐fold increased using the modified preparations. No interfering signals in the lower molecular weight range down to m/z 100 were observed, demonstrating the suitability of the method for small analytes. Crude solutions of commercial drugs, such as Aspirin complex® and IbuHEXAL® could be directly investigated without additional sample preparation.
CONCLUSIONS

Chemically modified diatom cell walls represent a powerful tool to support ionization in LDI MS. The lack of background signals in the low molecular weight region of the mass spectra allows also the investigations of small analytes.

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 Dates: 2014-04-232014-07-152014
 Publication Status: Published in print
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 Identifiers: Other: MS167
DOI: 10.1002/rcm.6930
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Title: Rapid Communications in Mass Spectrometry
  Other : Rapid Commun. Mass Spectrom.
Source Genre: Journal
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Publ. Info: New York, NY : John Wiley & Sons
Pages: - Volume / Issue: 28 (13) Sequence Number: - Start / End Page: 1521 - 1529 Identifier: ISSN: 0951-4198
CoNE: https://pure.mpg.de/cone/journals/resource/954925574961