English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics.

Hornburg, D., Drepper, C., Butter, F., Meissner, F., Sendtner, M., & Mann, M. (2014). Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics. Molecular & cellular proteomics: MCP, 13(12), 3410-3420. doi:10.1074/mcp.M113.037291.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Hornburg, Daniel1, Author              
Drepper, Carsten2, Author
Butter, Falk1, Author              
Meissner, Felix1, Author              
Sendtner, Michael2, Author
Mann, Matthias1, Author              
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: The fatal neurodegenerative disorders amyotrophic lateral sclerosis and spinal muscular atrophy are, respectively, the most common motoneuron disease and genetic cause of infant death. Various in vitro model systems have been established to investigate motoneuron disease mechanisms, in particular immortalized cell lines and primary neurons. Using quantitative mass-spectrometry-based proteomics, we compared the proteomes of primary motoneurons to motoneuron-like cell lines NSC-34 and N2a, as well as to non-neuronal control cells, at a depth of 10,000 proteins. We used this resource to evaluate the suitability of murine in vitro model systems for cell biological and biochemical analysis of motoneuron disease mechanisms. Individual protein and pathway analysis indicated substantial differences between motoneuron-like cell lines and primary motoneurons, especially for proteins involved in differentiation, cytoskeleton, and receptor signaling, whereas common metabolic pathways were more similar. The proteins associated with amyotrophic lateral sclerosis also showed distinct differences between cell lines and primary motoneurons, providing a molecular basis for understanding fundamental alterations between cell lines and neurons with respect to neuronal pathways with relevance for disease mechanisms. Our study provides a proteomics resource for motoneuron research and presents a paradigm of how mass-spectrometry-based proteomics can be used to evaluate disease model systems. 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

Details

show
hide
Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 25193168
DOI: 10.1074/mcp.M113.037291
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular & cellular proteomics : MCP
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 13 (12) Sequence Number: - Start / End Page: 3410 - 3420 Identifier: ISSN: 1535-9484