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  pH- and sodium-induced changes in a sodium/proton antiporter

Paulino, C., & Kühlbrandt, W. (2014). pH- and sodium-induced changes in a sodium/proton antiporter. eLife, 3: e01412. doi:10.7554/eLife.01412.

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 Creators:
Paulino, Cristina1, Author           
Kühlbrandt, Werner1, Author           
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              

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Free keywords: Na+/H+ antiporter; conformational changes; electron cryo-microscopy; electron crystallography; membrane protein structure; transport mechanism.
 Abstract: We examined substrate-induced conformational changes in MjNhaP1, an archaeal electroneutral Na+/H+-antiporter resembling the human antiporter NHE1, by electron crystallography of 2D crystals in a range of physiological pH and Na+ conditions. In the absence of sodium, changes in pH had no major effect. By contrast, changes in Nav concentration caused a marked conformational change that was largely pH-independent. Crystallographically determined, apparent dissociation constants indicated ∼10-fold stronger Na+ binding at pH 8 than at pH 4, consistent with substrate competition for a common ion-binding site. Projection difference maps indicated helix movements by about 2 Å in the 6-helix bundle region of MjNhaP1 that is thought to contain the ion translocation site. We propose that these movements convert the antiporter from the proton-bound, outward-open state to the Na+-bound, inward-open state. Oscillation between the two states would result in rapid Na+/H+ antiport

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Language(s): eng - English
 Dates: 2013-08-222013-12-112014-01-28
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.7554/eLife.01412
PMID: 24473071
PMC: 3900740
 Degree: -

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Title: eLife
Source Genre: Journal
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Affiliations:
Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 3 Sequence Number: e01412 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X