非表示:
キーワード:
Peptides and proteins; Cluster structure; Metal clusters; Cavities; Cluster chemistry
要旨:
Some N2-fixing bacteria prolong the functionality of nitrogenase in molybdenum starvation by a special Mo storage protein (MoSto) that can store more than 100 Mo atoms. The presented 1.6 Å X-ray structure of MoSto from Azotobacter vinelandii reveals various discrete polyoxomolybdate clusters, three covalently and three noncovalently bound Mo8, three Mo5−7, and one Mo3 clusters, and several low occupied, so far undefinable clusters, which are embedded in specific pockets inside a locked cage-shaped (αβ)3 protein complex. The structurally identical Mo8 clusters (three layers of two, four, and two MoOη octahedra) are distinguishable from the [Mo8O26]4− cluster formed in acidic solutions by two displaced MoOη octahedra implicating three kinetically labile terminal ligands. Stabilization in the covalent Mo8 cluster is achieved by Mo bonding to Hisα156−Nε2 and Gluα129−Oε1. The absence of covalent protein interactions in the noncovalent Mo8 cluster is compensated by a more extended hydrogen-bond network involving three pronounced histidines. One displaced MoOη octahedron might serve as nucleation site for an inhomogeneous Mo5−7 cluster largely surrounded by bulk solvent. In the Mo3 cluster located on the 3-fold axis, the three accurately positioned His140−Nε2 atoms of the α subunits coordinate to the Mo atoms. The formed polyoxomolybdate clusters of MoSto, not detectable in bulk solvent, are the result of an interplay between self- and protein-driven assembly processes that unite inorganic supramolecular and protein chemistry in a host−guest system. Template, nucleation/protection, and catalyst functions of the polypeptide as well as perspectives for designing new clusters are discussed.
