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  The ESFRI Instruct Core Centre Frankfurt: automated high-throughput crystallization suited for membrane proteins and more

Thielmann, Y., Koepke, J., & Michel, H. (2012). The ESFRI Instruct Core Centre Frankfurt: automated high-throughput crystallization suited for membrane proteins and more. Special Issue: International Conference on Structural Genomics, 2011, 63-69.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-D564-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-D565-6
Genre: Journal Article

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 Creators:
Thielmann, Yvonne1, Author              
Koepke, Juergen1, Author              
Michel, Hartmut1, Author              
Affiliations:
1Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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Free keywords: Calorimetry; Crystallization; High-throughput; Mass spectrometry; Membrane protein; X-ray diffraction
 Abstract: Abstract Structure determination of membrane proteins and membrane protein complexes is still a very challenging field. To facilitate the work on membrane proteins the Core Centre follows a strategy that comprises four labs of protein analytics and crystal handling, covering mass spectrometry, calorimetry, crystallization and X-ray diffraction. This general workflow is presented and a capacity of 20% of the operating time of all systems is provided to the European structural biology community within the ESFRI Instruct program. A description of the crystallization service offered at the Core Centre is given with detailed information on screening strategy, screens used and changes to adapt high throughput for membrane proteins. Our aim is to constantly develop the Core Centre towards the usage of more efficient methods. This strategy might also include the ability to automate all steps from crystallization trials to crystal screening; here we look ahead how this aim might be realized at the Core Centre.

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Language(s): eng - English
 Dates: 2012-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 611798
 Degree: -

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Title: Special Issue: International Conference on Structural Genomics, 2011
Source Genre: Issue
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 63 - 69 Identifier: -

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Title: Journal of Structural and Functional Genomics
  Alternative Title : J. Struct. Funct. Genomics
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 13 (2) Sequence Number: - Start / End Page: - Identifier: -